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Horm Metab Res 1995; 27(12): 523-526
DOI: 10.1055/s-2007-980017
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© Georg Thieme Verlag Stuttgart · New York

Hepatic Lipase Secretion in the Human Hepatoblastoma Cell Line Hep G2 is not Related to Cellular Cholesterol Homeostasis

Anna-Lena Berg2 , C.-H. Florén1 , P. Nilsson-Ehle3
  • 1Department of Medicine, Malmö General Hospital, University of Lund, Malmö, Sweden
  • 2Departments of Nephrology, University Hospital, Lund, Sweden
  • 3Clinical Chemistry, University Hospital, Lund, Sweden
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Publication History

1992

1995

Publication Date:
23 April 2007 (online)

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Abstract

The human hepatoblastoma cell line Hep G2 releases the enzyme hepatic lipase during incubation with heparin. In this study, hepatic lipase activity was released by low concentrations of heparin, and the release was linear with time for up to about 10 hours. Preincubations of cells with LDL or compactin induced marked but differential changes in hepatic lipase secretion, cellular cholesterol content and low density lipoprotein receptor activity, suggesting that the secretion of hepatic lipase is regulated independently of cholesterol homeostasis.

Key words

Hepatic Lipase - Human Hepatoblastoma Cell Line (Hep G2) - LDL Receptor - Cellular Cholesterol

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