Horm Metab Res 1980; 12(7): 300-303
DOI: 10.1055/s-2007-996275

© Georg Thieme Verlag, Stuttgart · New York

Insulin Binding to Liver Cells: A Simple and Useful In Vivo Model

J. J. Gagliardino1 , M. T. Pessacq1 , D. Turyn2 , J. M. Dellacha2
  • 1Centro de Endocrinología Experimental y Aplicada (CENEXA), UNLP-CONICET: Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
  • 2Departamento de Química Biológica. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires and Centro para el Estudio de las Hormonas Hipofisarias, Buenos Aires, Argentina
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Publication History



Publication Date:
14 March 2008 (online)


Insulin binding to rat liver cells was studied in vivo, by I.V. injection of (125I)-labelled hormone either alone or together with different amounts of unlabelled insulin.

Native insulin competes with the tracer in a manner similar to the one observed in the in vitro preparation. Prolactin, bovine growth hormone or glucagon were unable to displace labelled insulin from its binding sites.

The hypoglycemic effect of various amounts of native insulin was measured and correlated to the binding studies.

It was found that maximal insulin-induced hypoglycemia occurs at an insulin concentration at which only about 12 % of the measurable receptors are occupied.