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Horm Metab Res 1980; 12(7): 314-317
DOI: 10.1055/s-2007-996278
ORIGINALS

© Georg Thieme Verlag, Stuttgart · New York

Immunoreactive Luteinizing Hormone-Releasing Hormone (LHRH) in Ovine Pineal Glands

J. E. Wheaton
  • Department of Animal Science, University of Minnesota, St. Paul, Minnesota, U.S.A.
Scientific Journal Series Paper Number 10,658 of the Minnesota Agricultural Experiment Station, St. Paul
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Publication History

1979

1979

Publication Date:
14 March 2008 (online)

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Summary

Ovine pineal glands (PGs, n = 168), cerebral cortex (C) and stalk-median eminences (SMEs) were obtained fresh from slaughterhouse sheep and mature ewes during the estrous cycle. Tissues were extracted in 2N acetic acid (aa) followed by either glacial aa or ethanol. Portions of the preparations were further subjected to gel filtration using Sephadex G-25. Neutralized extracts were assayed for LHRH using radioimmunoassay. LHRH immunoreactivity was consistantly detected in the various extracts, ranging from 7-150 pg/PG (0.1-1.7 pg/mg), 0.1-0.3 pg/mg C and 35-72 ng/SME (0.7-1.4 ng/mg). Slopes of binding displacement regression lines for the preparations were not different from that of synthetic LHRH. Chromatographic elution profiles for preparations of SMEs and PGs were similar and the bulk of immunoreactivity eluted in a position coincident with that of synthetic LHRH. Immunoreactivity in cortical extracts ran in advance of the others. PG contents of LHRH immunoreactivity were similar during the estrous cycle.

Key-Words

Pineal Glands - LHRH - Sheep

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