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Exp Clin Endocrinol Diabetes 2008; 116: S13-S20
DOI: 10.1055/s-2008-1080898
Review

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Proteomic Profiling of β-cells Using a Classical Approach – Two-dimensional Gel Electrophoresis

C. Süß 1 , M. Solimena 1 , 2 , 3
  • 1Experimental Diabetology, Dresden University of Technology, Dresden, Germany
  • 2Department of Internal Medicine III, Dresden University of Technology, Dresden, Germany
  • 3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Further Information

Publication History

received 15.04.2008 first decision 15.05.2008

accepted 15.05.2008

Publication Date:
05 September 2008 (online)

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Abstract

Proteomics has rapidly become a major field of research in biology and medicine. Its main aim is to obtain a global overview of the expression pattern of proteins and their relationship in any given condition of a biological system. This knowledge is of particular interest to elucidate the pathogenesis of complex disorders, such as diabetes. Separation of proteins by two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS) for their identification is perhaps the most widely used proteomic approach. In this review we have focused our attention on studies that have taken advantage of these methodologies to investigate the proteome of pancreatic islets, β-cells and insulinoma cells in different conditions. As β-cells of the pancreatic islets produce and secrete insulin, the main hormone for control of glucose homeostasis, these analyses may help to elucidate the mechanisms regulating insulin secretion and the development of various forms of diabetes, as well as to identify drug targets for therapeutic approaches.

Key words

beta cells - diabetes - autoimmunity - proteomics - glucose regulation - two-dimensional differential gel electrophoresis

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Correspondence

M. Solimena

Experimental Diabetology

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01307 Dresden

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Phone: +49/351/458 66 11

Fax: +49/351/458 63 30

Email: michele.solimena@tu-dresden.de

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