Thrombin generation in severe haemophilia A and B: the endogenous thrombin potential in platelet-rich plasma

Thomas Siegemund; Sirak Petros; Annelie Siegemund; Ute Scholz; Lothar Engelmann

doi:10.1160/TH03-01-0027

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 90(05): 781-786
DOI: 10.1160/TH03-01-0027

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Thrombin generation in severe haemophilia A and B: the endogenous thrombin potential in platelet-rich plasma

Authors

Thomas Siegemund

¹Clinical Haemostaseology, Medical Clinic I, University of Leipzig, Germany
Sirak Petros

¹Clinical Haemostaseology, Medical Clinic I, University of Leipzig, Germany
Annelie Siegemund

¹Clinical Haemostaseology, Medical Clinic I, University of Leipzig, Germany

²Laboratory Practice Dr. G. Reising-Ackermann, Leipzig, Germany
Ute Scholz

¹Clinical Haemostaseology, Medical Clinic I, University of Leipzig, Germany
Lothar Engelmann

¹Clinical Haemostaseology, Medical Clinic I, University of Leipzig, Germany

Abstract

Summary

Thrombin generation was investigated in platelet-rich plasma (PRP) from 11 healthy controls, 17 patients with severe haemophilia A and 7 patients with severe haemophilia B. Mean endogenous thrombin potential (ETP) in arbitrary fluorescence units (FU) was 226.9 ± 44.6, 186.4 ± 22.5, 154.2 ± 41.3 in controls, haemophilia A and B, respectively, all at a platelet count of 200 × 10⁹/l (p = 0.004 for controls vs.haemophilia A, p = 0.003 for controls vs. haemophilia B, no significant difference between haemophilia A and B). The contribution of FVIII to thrombin generation in haemophilia A was 1.31 ± 0.16 FU/% of FVIII:C activity , while for FIX in haemophilia B this was 0.80 ± 0.21 FU/% of FIX activity. There was an almost linear relationship between increasing platelet count and thrombin generation up to a mean platelet count of 100 × 10⁹/l. Further increase in platelet count has only a marginal influence on thrombin generation. Platelets increase ETP in haemophilia A by 0.184 ± 0.022 FU/10⁹platelets/l and in haemophilia B by 0.319 ± 0.085 FU/10⁹platelets/l, and this was significantly different between the two groups (p = 0.0002). This influence of platelets diminishes with increasing concentration of either FVIII or FIX. In conclusion, there is a difference in thrombin generation between haemophilia A and B, and this may be attributed to the role of platelets in the assembly of the tenase complex on their surface.

Keywords

Thrombin generation - haemophilia - factor VIII - factor IX - platelets

Full Text

References

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