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Thromb Haemost 2006; 96(05): 562-567
DOI: 10.1160/TH06-03-0179
Theme Issue Article
Schattauer GmbH

Use of calibrated automated thrombinography ± thrombomodulin to recognise the prothrombotic phenotype

Yesim Dargaud
1   Unite d’Hemostase Clinique – Laboratoire d’Hemostase, Hopital Edouard Herriot, Lyon, France
2   Service d’Angiologie – Explorations Vasculaires, Hopital Edouard Herriot, Lyon, France
,
M. Christine Trzeciak
1   Unite d’Hemostase Clinique – Laboratoire d’Hemostase, Hopital Edouard Herriot, Lyon, France
,
Jean Claude Bordet
1   Unite d’Hemostase Clinique – Laboratoire d’Hemostase, Hopital Edouard Herriot, Lyon, France
,
Jacques Ninet
2   Service d’Angiologie – Explorations Vasculaires, Hopital Edouard Herriot, Lyon, France
3   Service de Médecine Interne, Hopital Edouard Herriot, Lyon, France
,
Claude Negrier
1   Unite d’Hemostase Clinique – Laboratoire d’Hemostase, Hopital Edouard Herriot, Lyon, France
› Author Affiliations
Further Information

Publication History

Received 28 March 2006

Accepted after resubmission 07 August 2006

Publication Date:
01 December 2017 (online)

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Summary

There is currently no validated method to detect a prothrombotic phenotype. The question remains, can tissue factor (TF) induced thrombin generation (TG), as measured with the calibrated automated thrombinography (CAT) technique, according to Hemker et al., recognise a prothrombotic state either as such, or when the activated proteinC (APC)-system is boosted with thrombomodulin (TM)? We determined the normal range of CAT-TG ± TM in a group of 71 healthy blood donors, in 11 healthy women using oral contraceptives (OC), and in 89 patients with a history of venous thromboembolism (VTE), divided into a group of 50 in whicha prothrombotic risk factor could be found (VTEprf+) and 39 others (VTEprf-). The endogenous thrombin potential (ETP) in the OC, VTEprf+ and VTEprf-group was significantly higher than for the controls. In the presence of TM, the differences were significantly higher than in its absence. The VTEprf+ group had a higher ETP, ± TM than the VTEprf group. In conclusion,TG, measured with the CAT technique in the presence of TM is capable of detecting the prothrombotic phenotype with a high sensitivity of 0.93 (95% confidence limits 0.82–0.99).

Keywords

Thrombin generation - thrombomodulin - hypercoagulability - calibrated automated thrombogram,venous thromboembolism
 

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