Transendothelial migration drives dissociation of plateletmonocyte complexes

Janine M. van Gils; Paula A. da Costa Martins; Anita Mol; Peter L. Hordijk; Jaap Jan Zwaginga

doi:10.1160/TH08-03-0165

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 100(02): 271-279
DOI: 10.1160/TH08-03-0165

Platelets and Blood Cells

Schattauer GmbH

Transendothelial migration drives dissociation of plateletmonocyte complexes

Authors

Janine M. van Gils

¹Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Paula A. da Costa Martins

²Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Anita Mol

¹Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Peter L. Hordijk

¹Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Jaap Jan Zwaginga

²Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands

³Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands

Abstract

Summary

Monocytes and platelets are both crucially involved in atherogenesis. Importantly, activated platelets bound to circulating monocytes increase adhesion of the monocytes and thus mediate colocalization of both cell types at the vessel wall. We examined the fate of the platelets upon migration of these potentially pro-atherogenic platelet-monocyte complexes (PMC) across activated endothelium. Platelet-monocyte complex migration was studied both quantitatively by means of Transwell filters coated with endothelial cells, as well as qualitatively with different imaging techniques, and in the absence or presence of flow. Upon PMC transendothelial migration, platelets relocate with monocytic P-selectin glycoprotein ligand-1 (PSGL-1) to the rear of the monocyte, detach,and remain at the endothelial surface. Platelet dissociation appeared not to be due to reduced PSGL-1 expression or reduced platelet-binding capacity of the migrated monocytes. In addition, different endothelial matrix proteins with different platelet-binding capacities coated on the Transwell filter, instead of endothelial cells, did not affect PMC dissociation. In contrast, lowering the mechanical stress that PMC experience during transmigration prevented dissociation of platelets. In conclusion, PMC dissociate during transendothelial migration as a result of monocytic PSGL-1 redistribution and mechanical stress. PMC-mediated deposition of activated platelets at sites of vascular inflammation is likely relevant for cardiovascular disease progression or vascular regeneration.

Keywords

Monocytes - platelet immunology - cell migration - endothelial cells

Full Text

References

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