Thromb Haemost 2009; 102(05): 874-878
DOI: 10.1160/TH08-12-0797
Theme Issue Article
Schattauer GmbH

The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids

Timothy R. Rudd*
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Mark A. Skidmore*
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Scott E. Guimond
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Joseph Holman
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Jeremy E. Turnbull
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Robert M. Lauder
2  School of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Bailrigg, Lancaster, UK
,
David G. Fernig
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
,
Edwin A. Yates
1  School of Biological Sciences, University of Liverpool, Liverpool, UK
› Author Affiliations
Further Information

Publication History

Received: 08 December 2008

Accepted after major revision: 01 February 2009

Publication Date:
27 November 2017 (online)

Summary

The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 and 260 nm. Principal component analysis of the spectra allowed their differentiation into a number of groups related to the means of their production reflecting the structural changes introduced by each process. The findings suggest that CD provides a complementary technique for the rapid analysis of heparin preparations.

* Joint first authorship.