Abnormal megakaryopoiesis and platelet function in cyclooxygenase-2-deficient mice

Silvia S. Barbieri; Giovanna Petrucci; Eva Tarantino; Patrizia Amadio; Bianca Rocca; Maurizio Pesce; Kellie R. Machlus; Franco O. Ranelletti; Sara Gianellini; Babette B. Weksler; Joseph E. Italiano Jr.; Elena Tremoli

doi:10.1160/TH14-10-0872

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2015; 114(06): 1218-1229
DOI: 10.1160/TH14-10-0872

Blood Cells, Inflammation and Infection

Schattauer GmbH

Abnormal megakaryopoiesis and platelet function in cyclooxygenase-2-deficient mice

Silvia S. Barbieri

¹Centro Cardiologico Monzino, IRCCS, Milan, Italy

,

Giovanna Petrucci^*

²Institute of Pharmacology, Catholic University School of Medicine, Rome, Italy

,

Eva Tarantino^*

³Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy

,

Patrizia Amadio

¹Centro Cardiologico Monzino, IRCCS, Milan, Italy

,

Bianca Rocca

²Institute of Pharmacology, Catholic University School of Medicine, Rome, Italy

,

Maurizio Pesce

¹Centro Cardiologico Monzino, IRCCS, Milan, Italy

,

Kellie R. Machlus

⁴Hematology Division, Department of Medicine, Brigham and Women’s Hospital, Boston, Massachusetts, USA

⁵Harvard Medical School, Boston, Massachusetts, USA

,

Franco O. Ranelletti

⁶Department of Histology, Catholic University School of Medicine, Rome, Italy

,

Sara Gianellini

¹Centro Cardiologico Monzino, IRCCS, Milan, Italy

,

Babette B. Weksler

⁷Division of Hematology-Medical Oncology, Weill Cornell Medical College, New York, New York, USA

,

Joseph E. Italiano Jr.

⁴Hematology Division, Department of Medicine, Brigham and Women’s Hospital, Boston, Massachusetts, USA

⁵Harvard Medical School, Boston, Massachusetts, USA

⁸Vascular Biology Program, Department of Surgery, Children’s Hospital, Boston, Massachusetts, USA

,

Elena Tremoli

¹Centro Cardiologico Monzino, IRCCS, Milan, Italy

³Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy

› Institutsangaben

Abstract

Summary

Previous studies suggest that cyclooxygenase-2 (COX-2) might influence megakaryocyte (MK) maturation and platelet production in vitro. Using a gene deletion model, we analysed the effect of COX-2 deficiency on megakaryopoiesis and platelet function. COX-2^-/- mice (10–12 weeks old) have hyper-responsive platelets as suggested by their enhanced aggregation, TXA₂ biosynthesis, CD62P and CD41/CD61 expression, platelet-fibrinogen binding, and increased thromboembolic death after collagen/epinephrine injection compared to wild-type (WT). Moreover, increased platelet COX-1 expression and reticulated platelet fraction were observed in COX-2^-/- mice while platelet count was similar to WT. MKs were significantly reduced in COX-2^-/- bone marrows (BMs), with high nuclear/cytoplasmic ratios, low ploidy and poor expression of lineage markers of maturation (CD42d, CD49b). However, MKs were significantly increased in COX-2^-/- spleens, with features of MK maturation markers which were not observed in MKs of WT spleens. Interestingly, the expression of COX-1, prostacyclin and PGE₂ synthases and prostanoid pattern were modified in BMs and spleens of COX-2^-/- mice. Moreover, COX-2 ablation reduced the percentage of CD49b⁺ cells, the platelet formation and the haematopoietic stem cells in bone marrow and increased their accumulation in the spleen. Splenectomy decreased peripheral platelet number, reverted their hyper-responsive phenotype and protected COX-2^-/- mice from thromboembolism. Interestingly, fibrosis was observed in spleens of old COX-2^-/- mice (28 weeks old). In conclusion, COX-2 deletion delays BM megakaryopoiesis promoting a compensatory splenic MK hyperplasia, with a release of hyper-responsive platelets and increased thrombogenicity in vivo. COX-2 seems to contribute to physiological MK maturation and pro-platelet formation.

Keywords

COX-2 - platelets - thrombosis - megakaryocytes

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Referenzen

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