Zinc promotes clot stability by accelerating clot formation and modifying fibrin structure

Sara J. Henderson; Jing Xia; Huayin Wu; Alan R. Stafford; Beverly A. Leslie; James C. Fredenburgh; David A. Weitz; Jeffrey I. Weitz

doi:10.1160/th15-06-0462

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2016; 115(03): 533-542
DOI: 10.1160/th15-06-0462

Coagulation and Fibrinolysis

Schattauer GmbH

Zinc promotes clot stability by accelerating clot formation and modifying fibrin structure

Authors

Sara J. Henderson

¹Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada

²Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
Jing Xia

⁵School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
Huayin Wu

⁵School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
Alan R. Stafford

¹Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada

³Department of Medicine, McMaster University, Hamilton, Ontario, Canada
Beverly A. Leslie

¹Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada

³Department of Medicine, McMaster University, Hamilton, Ontario, Canada
James C. Fredenburgh

¹Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada

³Department of Medicine, McMaster University, Hamilton, Ontario, Canada
David A. Weitz

⁵School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA
Jeffrey I. Weitz

¹Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada

²Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada

³Department of Medicine, McMaster University, Hamilton, Ontario, Canada

⁴Department of Medical Sciences, McMaster University, Hamilton, Ontario, Canada

Abstract

Summary

Zinc released from activated platelets binds fibrin(ogen) and attenuates fibrinolysis. Although zinc also affects clot formation, the mechanism and consequences are poorly understood. To address these gaps, the effect of zinc on clot formation and structure was examined in the absence or presence of factor (F) XIII. Zinc accelerated a) plasma clotting by 1.4-fold, b) fibrinogen clotting by 3.5- and 2.3-fold in the absence or presence of FXIII, respectively, c) fragment X clotting by 1.3-fold, and d) polymerisation of fibrin monomers generated with thrombin or batroxobin by 2.5– and 1.8-fold, respectively. Whereas absorbance increased up to 3.3-fold when fibrinogen was clotted in the presence of zinc, absorbance of fragment X clots was unaffected by zinc, consistent with reports that zinc binds to the αC-domain of fibrin(ogen). Scanning electron microscopic analysis revealed a twofold increase in fibre diameter in the presence of zinc and in permeability studies, zinc increased clot porosity by 30-fold with or without FXIII. Whereas FXIII increased clot stiffness from 128 ± 19 Pa to 415 ± 27 Pa in rheological analyses, zinc reduced clot stiffness by 10– and 8.5-fold in the absence and presence of FXIII, respectively. Clots formed in the presence of zinc were more stable and resisted rupture with or without FXIII. Therefore, zinc accelerates clotting and reduces fibrin clot stiffness in a FXIII-independent manner, suggesting that zinc may work in concert with FXIII to modulate clot strength and stability.

Keywords

Zinc - fibrinogen - fibrin - FXIII - clot structure

Full Text

References

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