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Tierarztl Prax Ausg K Kleintiere Heimtiere 2017; 45(02): 77-83
DOI: 10.15654/TPK-160366
Originalartikel
Schattauer GmbH

Nervenverteilung und -verteilungsdichte in der Hüftgelenkskapsel des Hundes

Vergleichende Untersuchung gesunder und dysplastischer HüftgelenkeNerve distribution and density in the canine hip joint capsule. Comparison of healthy and dysplastic hip joints
Felix Giebels
1   Tierärztliche Praxis für Kleintiere PD Dr. med. vet. S. Kinzel, Aachen
,
Andreas Prescher
2   Institut für Molekulare und Zelluläre Anatomie, RWTH Aachen, Prosektur, Aachen
,
Stefan Wagenpfeil
3   Institut für Medizinische Biometrie, Epidemiologie und Medizinische Informatik, Universität des Saarlandes, Homburg/Saar
,
Arno Bücker
4   Klinik für Diagnostische und Interventionelle Radiologie, Universität des Saarlandes, Homburg/Saar
,
Sylvia Kinzel
1   Tierärztliche Praxis für Kleintiere PD Dr. med. vet. S. Kinzel, Aachen
› Author Affiliations
Further Information

Publication History

Eingegangen: 26 March 2016

Akzeptiert nach Revision: 02 February 2016

Publication Date:
08 January 2018 (online)

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Zusammenfassung

Gegenstand und Ziel: Die Hüftgelenkskapsel unterliegt bei der Hüftgelenksdysplasie (HD) erhöhten Zugkräften, die zu einer Entzündung des Kapselgewebes führen. Eine entzündungsassoziierte Zunahme der Nervenverteilungsdichte wurde in verschiedenen Organen nachgewiesen und könnte auch im Rahmen einer HD Ursache für Hüftgelenksschmerz und Koxarthrose sein. Ziel dieser Studie war, bei Hunden mit normo- bzw. dysplastischen Hüftgelenken Unterschiede in der Nervenverteilungsdichte innerhalb der Hüftgelenkskapsel aufzuzeigen. Material und Methoden: Bei 16 aus anderen Gründen euthanasierten Labrador Retrievern erfolgte anhand des radiologischen Befunds eine Kate gorisierung der Hüftgelenke als normoplastisch (Gruppe 1, n = 18) oder dysplastisch (Gruppe 2, n = 14). Die histologischen Präparate der Hüftgelenkskapseln wurden nach Färbung der Nerven fasern mit der Sihler-Färbemethode eingescannt und mittels einer Matrix in 10 gleich große Quadranten, nummeriert von dorsomedial (Q01) nach kraniodorsolateral (Q10), unterteilt. Durch Berechnung des prozentualen Anteils schwarzer Pixel mittels halbautomatischer Bildanalyse wurde die Nervenverteilungsdichte für jeweils das gesamte Präparat und die einzelnen Quadranten analysiert. Der statistische Vergleich erfolgte mittels t-Test für unabhängige Stichproben. Ergebnisse: Bei Hunden mit HD zeigte sich im kraniodorsolateralen Anteil der Hüftgelenkskapsel eine signifikant höhere Nervenverteilungsdichte (p = 0,03). Die mittlere Nervenverteilungsdichte für die gesamte Hüftgelenkskapsel differierte zwischen den beiden Gruppen nicht signifikant. Schlussfolgerung: Eine erhöhte Nervenverteilungsdichte im kraniodorsalen Anteil der Hüftgelenkskapsel bei Hunden mit HD könnte Folge der großen Zugkräfte in diesem Bereich und eine Ursache für die mit der HD einhergehende Schmerzhaftigkeit und Koxarthrose sein. Klinische Relevanz: Die Ergebnisse liefern die pathophysiologische Grundlage für den Erfolg der Deperiostierung des kranialen Azetabulumrandes im Rahmen einer Hüftgelenksdenervation zur Erzielung einer suffizienten Schmerzlinderung und Entzündungsreduktion bei Hunden mit HD.

Summary

Objective: The hip-joint capsule is exposed to increased tension forces during canine hip dysplasia, resulting in inflammation of the capsular tissue. It has been postulated that inflammation is associated with an increased nerve-distribution density. Therefore, it could be supposed that the nerve-distribution density in the hip-joint capsule is higher in dogs with dysplastic hip compared to healthy dogs. Material and methods: In 16 Labrador Retriever dogs that had been euthanised due to unrelated reasons, the hip joints were classified as normoplastic (group 1, n = 18) or dysplastic (group 2, n = 14) based on radiography. Following staining of the capsular nerve fibres by the Sihler method, histological specimens of the hip-joint capsules were scanned. By subdividing each specimen into 10 quadrants numbered from dorsomedial (Q01) to craniodorsolateral (Q10), the ratio of black to white pixels was calculated digitally for each specimen and each quadrant by using a semiautomatic image analysis. Statistical analysis was performed using an independent t-test. Results: Comparison of the mean values of each quadrant showed a significantly higher (p < 0.03) nerve distribution density for the craniodorsolateral quadrant (Q10) in group 2 when compared to group 1. Mean nerve-distribution density for all quadrants combined was not significantly different between the two groups. Conclusion: The increase in nervedistribution density of the craniodorsal region of the hip-joint capsule in dogs with dysplastic hip could be the result of increased tension forces on this area following hip-joint dysplasia. The craniodorsal region of the hip-joint capsule is an important origin of pain and coxarthrosis in canine hip dysplasia. Clinical relevance: The results provide the pathophysiological basis for the efficacy of hip-joint denervation. Denervation of the cranial region of the acetabular rim is essential to reduce capsular inflammation and joint-related pain in canine hip dysplasia.

Schlüsselwörter

Neurogene Entzündung - Denervation - Koxarthrose - Kapseldehnung

Keywords

Neurogenic inflammation - denervation - coxarthrosis - capsular stretch
 

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