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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2021; 79(03): 189-194
DOI: 10.1590/0004-282X-ANP-2020-0195
ARTICLE

Increased serum chemerin levels associated with carotid intima-media thickness

Aumento dos níveis séricos de chemerin associados à espessura das camadas intima-media carotídea
Caner Feyzi DEMİR 
1   Firat University, Faculty of Medicine, Department of Neurology, Elazig, Turkey.
,
İklimya Nimet ATAŞ 
2   Health Sciences University, Elazig Fethi Sekin City Hospital, Department of Neurology, Elazig, Turkey.
,
Ferhat BALGETİR 
1   Firat University, Faculty of Medicine, Department of Neurology, Elazig, Turkey.
,
Hakan ARTAŞ 
3   Firat University, Faculty of Medicine, Department of Radiology, Elazig, Turkey.
,
Murat GÖNEN 
1   Firat University, Faculty of Medicine, Department of Neurology, Elazig, Turkey.
,
Süleyman AYDIN 
4   Firat University, Faculty of Medicine, Department of Medical Biochemistry and Clinical Biochemistry, Elazig, Turkey.
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ABSTRACT

Background: Elevated levels of chemerin can predict future ischemic cerebrovascular disease. Although chemerin is thought to play a role in atherosclerotic inflammation, whether circulating chemerin levels are associated with the severity of atherosclerosis remains to be determined. Objectives: Through the use of carotid Doppler ultrasonography, our aim in this study was to investigate the relationships of serum chemerin levels with carotid intima-media thickness (CIMT) as an indicator of generalized atherosclerosis. Methods: This study compared 40 patients with ischemic stroke and 40 healthy subjects. Measurements were made at end-diastole using color Doppler ultrasonography (CDUS) after a 5-min rest interval in a quiet and dark room. CIMT was defined as the distance between the innermost edge of the luminal echo to the innermost edge of the media/adventitia echo. CIMT was measured in the posterior wall of both common carotid arteries within 1 cm proximally to the bulbus. Three measurements were made on both sides and the average measurement was taken as the CIMT. Serum chemerin levels were determined in all patients and healthy subjects. Results: Serum chemerin levels were significantly higher in the patient group than in the control group (p=0.004). Serum chemerin levels were positively correlated with CIMT (p<0.05). There was a significant difference between the groups with regard to CIMT (p<0.001). Conclusion: Elevated serum chemerin levels appear to be associated with CIMT, thus suggesting that a link exists between chemerin and atherosclerotic ischemic cerebrovascular disease.

RESUMO

Introdução: Níveis elevados de chemerin podem prever doenças cerebrovasculares isquêmicas futuras. Embora se acredite que a chemerin desempenhe um papel na inflamação aterosclerótica, ainda não foi determinado se os níveis circulantes de chemerin estão associados à gravidade da aterosclerose Objetivos: Por meio do uso da ultrassonografia Doppler da carótida, nosso objetivo neste estudo foi investigar as relações dos níveis séricos de chemerin com a espessura da íntima-média da carótida (EIMC) como um indicador de aterosclerose generalizada. Métodos: Este estudo comparou 40 pacientes com AVC isquêmico e 40 indivíduos saudáveis. As medidas foram feitas no final da diástole usando ultrassonografia Doppler em cores (USDC), após um intervalo de descanso de 5 minutos em um quarto silencioso e escuro. A EIMC foi definida como a distância entre a borda mais interna do eco luminal e a borda mais interna do eco da mídia/adventícia. EIMC foi medido na parede posterior de ambas as artérias carótidas comuns dentro de 1 cm proximalmente ao bulbo. Três medições foram feitas em ambos os lados e a medição média foi tomada como o EIMC. Os níveis séricos de chemerin foram determinados em todos os pacientes e indivíduos saudáveis. Resultados: Os níveis séricos de chemerin foram significativamente maiores no grupo de pacientes do que no grupo controle (p=0,004). Os níveis séricos de chemerin foram positivamente correlacionados com EIMC (p<0,05). Houve diferença significativa entre os grupos em relação à EIMC (p<0,001). Conclusão: Níveis séricos elevados de chemerin parecem estar associados com a EIMC, sugerindo que existe uma ligação entre chemerin e doença cerebrovascular isquêmica aterosclerótica.

Keywords:

Chemerin Protein - Stroke - Carotid Intima-Media Thickness

Palavras-chave:

Chemerin - Acidente Vascular Cerebral - Espessura Intima-Media Carotídea

Authors’ contributions:

CFD: developed the study idea, collected the data, participated in the statistical calculations and wrote the manuscript. İNA: collected the data, participated in the statistical calculations and approved the final manuscript. FB: participated in the statistical calculations, was involved in literature search and wrote the manuscript. HA: developed the study idea, made and evaluated doppler ultrasounds and participated in the statistical calculations. MG: participated in the statistical calculations, analyzed the data and approved the final manuscript. SA: developed the study idea, analyzed the data and approved the final manuscript.




Publication History

Received: 05 May 2020

Accepted: 27 July 2020

Article published online:
07 June 2023

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  • REFERENCES

  • 1 Kural E. İnme epidemiyolojisi Balkan S. Serebrovasküler Hastalıklar. 2. Baskı: Güneş Kitabevi Ltd Şti. Türkiye; 2002. p.39-40.
  • 2 Biller J, Love BB. Vascular Disease of the Nervous System. In: Bradley WG, Daroff RB, Fenichel GM, Marsden CD, editors. Neurology in Clinical Practice. 5. ed. Oxford: Butterworth-Heinemann; 2008. p.1166-7.
  • 3 Taveras JM. Brain vascular disorders. In: Taveras JM, Neuroradiology. 3. ed. Baltimore: Williams and Wilkins; 1996. p.462-71.
  • 4 Pasterkamp G, Schoneveld AH, van der Wal AC, Hijnen DJ, van Wolveren WJ, Plomp S, et al. Inflammation of atherosclerotic cap and shoulder of the plaque is a common and locally observed feature in unruptured plaques of femoral and coronary arteries. Arterioscler Thromb Vasc Biol. 1999 Jan;19(1):54-8. https://doi.org/10.1161/01.atv.19.1.54
  • 5 Jander S, Sitzer M, Schumann R, Schroeter M, Siebler M, Steinmetz H, et al. Inflammation in high-grade carotid stenosis: a possible role for macrophages and T cells in plague destabilization. Stroke. 1998 Aug;29(8):1625-30. https://doi.org/10.1161/01.str.29.8.1625
  • 6 Ernst MC, Sinal CJ. Chemerin: at the crossroads of inflammation and obesity. Trends Endocrinol Metab. 2010 Nov;21(11):660-7. https://doi.org/10.1016/j.tem.2010.08.001.
  • 7 Wittamer V, Franssen JD, Vulcano M, Mirjolet JF, Le Poul E, Migeotte I, et al. Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Med. 2003 Oct;198(7):977-85. https://doi.org/10.1084/jem.20030382
  • 8 Wittamer V, Bondue B, Guillabert A, Vassart G, Parmentier M, Communi D. Neutrophil-mediated maturation of chemerin: a link between innate and adaptive immunity. J Immunol. 2005 Jul;175(1):487-93. https://doi.org/10.4049/jimmunol.175.1.487
  • 9 Merritt CRB. Physics of ultrasound. In: Rumack CM, Wilson S, Charboneau JM, Johnson J, editors. Diagnostic Ultrasound. 3. ed. St. Louis, Mo: Elsevier Mosby, 2005. p.885-916.
  • 10 Berkow R, Fletcher AJ, Chir B. The Merck Manual of Diagnosis and Therapy. 6. ed. New Jersey: Merck Research Laboratories; 1992. p.406-14.
  • 11 Koolpe HA. Vascular imaging: angiography and the new modalities. Radiol Clin North Am. 1986 Sep;24(3):337-525.
  • 12 Nikic P, Savic M, Jakovljevic V, Djuric D. Carotid atherosclerosis, coronary atherosclerosis and carotid intima-media thickness in patients with ischemic cerebral disease: Is there any link? Exp Clin Cardiol. Summer 2006;11(2):102-6.
  • 13 KiechI S, Willeit J. The natural course of atherosclerosis. Part I: incidence and progression. Arterioscler Thromb Vasc Biol. 1999 Jun;19(6):1484-90. https://doi.org/10.1161/01.atv.19.6.1484
  • 14 Gnasso A, Carallo C, Irace C, Spagnuolo V, De Novara G, Mattioli PL, et al. Association between intima-media thickness and wall shear stress in common carotid arteries in healthy male subjects. Circulation. 1996 Dec;94(12):3257-62. https://doi.org/10.1161/01.cir.94.12.3257
  • 15 Tada H, Nakagawa T, Okada H, Nakahashi T, Mori M, Sakata K, et al. Clinical impact of carotid plaque score rather than carotid intima-media thickness on recurrence of atherosclerotic cardiovascular disease events. J Atheroscler Thromb. 2020 Jan;27(1):38-46. https://doi.org/10.5551/jat.49551
  • 16 Skrzypczyk P, Kuźma-Mroczkowska E, Kułagowska J, Brzewski M, Okarska-Napierała M, Pańczyk-Tomaszewska M. Carotid intima-media thickness in children with idiopathic nephrotic syndrome: A single center cross-sectional study. Clin Nephrol. 2019 Jun;91(6):353-62. https://doi.org/10.5414/CN109617.
  • 17 Polak JF, Herrington D, O'Leary DH. Associations of edge-detected and manual-traced common carotid artery intima-media thickness with incident peripheral artery disease: The Multi-Ethnic Study of Atherosclerosis. Vasc Med. 2019 Aug;24(4):306-12. https://doi.org/10.1177/1358863X19835925
  • 18 Avci AY, Akkucuk MH, Torun E, Arikan S, Can U, Tekindal MA. Migraine and subclinical atherosclerosis: endothelial dysfunction biomarkers and carotid intima-media thickness: a case-control study. Neurol Sci. 2019 Apr;40(4):703-11. https://doi.org/10.1007/s10072-019-3710-5
  • 19 Campbell BCV, De Silva DA, Macleod MR, Coutts SB, Schwamm LH, Davis SM, et al. Ischaemic Stroke. Nat Rev Dis Primers. 2019 Oct;5(1):70. https://doi.org/10.1038/s41572-019-0118-8.
  • 20 Lombardo A, Biasucci LM, Lanza GA, Coli S, Silvestri P, Cianflone D, et al. Inflammation as a possible link between coronary and carotid plaque instability. Circulation. 2004 Jun;109(25):3158-63. https://doi.org/10.1161/01.CIR.0000130786.28008.56.
  • 21 Libby P. Inflammation in atherosclerosis. Nature. 2002 Dec;420:868-74. https://doi.org/10.1038/nature01323
  • 22 Trayhurn P, Wood IS. Adipokines: inflammation and the pleiotropic role of White adipose tissue. Br J Nutr. 2004 Sep;92(3):347-55. https://doi.org/10.1079/bjn20041213
  • 23 Nagpal S, Patel S, Jacobe H, DiSepio D, Ghosn C, Malhotra M, et al. Tazarotene-induced gene 2 (TIG2), a novel retinoid responsive gene in skin. J Invest Dermatol. 1997 Jul;109(1):91-5. https://doi.org/10.1111/1523-1747.ep12276660
  • 24 Zabel BA, Silverio AM, Butcher EC. Chemokine-like receptor 1 expression and chemerin-directed chemotaxis distinguish plasmacytoid from myeloid dendritic cells in human blood. J Immunol. 2005 Jan;174(1):244-51. https://doi.org/10.4049/jimmunol.174.1.244
  • 25 Kaur J, Adya R, Tan BK, Chen J, Randeva HS. Identification of chemerin receptor (ChemR23) in human endothelial cells: chemerin-induced endothelial angiogenesis. Biochem Biophys Res Commun. 2010 Jan;391(4):1762-8. https://doi.org/10.1016/j.bbrc.2009.12.150
  • 26 Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. 2007 Oct;148(10):4687-94. https://doi.org/10.1210/en.2007-0175
  • 27 Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Kitazawa R, et al. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett. 2008 Mar;582(5):573-8. https://doi.org/10.1016/j.febslet.2008.01.023
  • 28 Hart R, Greaves DR. Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5. J Immunol. 2010 Sep;185(6):3728-39. https://doi.org/10.4049/jimmunol.0902154
  • 29 Becker M, Rabe K, Lebherz C, Zugwurst J, Göke B, Parhofer KG, et al. Expression of human chemerin induces insulin resistance in the skeletal muscle but does not affect weight, lipid levels, and atherosclerosis in LDL receptor knockout mice on high-fat diet. Diabetes. 2010 Nov;59(11):2898-903. https://doi.org/10.2337/db10-0362
  • 30 Spiroglou SG, Kostopoulos CG, Varakis JN, Papadaki HH. Adipokines in periaortic and epicardial adipose tissue: differential expression and relation to atherosclerosis. J Atheroscler Thromb. 2010 Feb;17(2):115-30. https://doi.org/10.5551/jat.1735
  • 31 Cao JJ, Thach C, Manolio TA, Psaty BM, Kuller LH, Chaves PH, et al. C-reactive protein, carotid intima-media thickness, and incidence of ischemic stroke in the elderly: the Cardiovascular Health Study. N Engl J Med. 2011 Jul;365(3):213-21. https://doi.org/10.1056/NEJMoa1012592
  • 32 O'Leary DH, Polak JF, Kronmal RA, Manolio TA, Burke GL, Wolfson SK Jr. Carotid-artery intima and media thickness as a risk factor for myocardial infarction and stroke in older adults. Cardiovascular Health Study Collaborative Research Group. N Engl J Med. 1999 Jan;340(1):14-22. https://doi.org/10.1056/NEJM199901073400103
  • 33 Yoo HJ, Choi HY, Yang SJ, Kim HY, Seo JA, Kim SG, et al. Circulating chemerin level is independently correlated with arterial stiffness. Atheroscler Thromb. 2012;19(1):59-66;discussion 67-8. https://doi.org/10.5551/jat.9647
  • 34 Zhao D, Bi G, Feng J, Huang R, Chen X. Association of serum chemerin levels with acute ischemic stroke and carotid artery atherosclerosis in a Chinese population. Med Sci Monit. 2015 Oct;21:3121-8. https://doi.org/10.12659/msm.895866
  • 35 Delikan O, Petek BB, Özer F, Akdağ G. İskemik inmede karotis intima-media kalınlığı ve homosistein arasındaki ilişki. Noro Psikiyatr Ars. 2012;49(1):53-8.