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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2016; 74(05): 367-372
DOI: 10.1590/0004-282X20160051
ARTICLE

A histomorphometric study of unmyelinated fibers of the fibular nerve in Wistar rats

Histomorfometria das fibras amielínicas do nervo fibular em ratos Wistar
Vânia Tognon-Miguel
1   Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Neurociências e Ciências do Comportamento, Ribeirão Preto SP, Brasil;
,
Adriana Helena do Nascimento-Elias
1   Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Neurociências e Ciências do Comportamento, Ribeirão Preto SP, Brasil;
2   Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Biomecânica, Medicina e Reabilitação do Aparelho Locomotor, Ribeirão Preto SP, Brasil.
,
Maria Cristina Lopes Schiavoni
1   Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Neurociências e Ciências do Comportamento, Ribeirão Preto SP, Brasil;
,
Amilton Antunes Barreira
1   Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Neurociências e Ciências do Comportamento, Ribeirão Preto SP, Brasil;
› InstitutsangabenSupport: FAPESP (Processo: 2012/11012-7), CNPq (Processo: 141524/2015-4) and FAEPA (Fundação de Apoio ao Ensino, Pesquisa e à Assistência do Hospital das Clínicas da Universidade de São Paulo).
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Erratum zu diesem Artikel:

ErratumArq Neuropsiquiatr 2016; 74(06): 1-1
DOI: 10.1590/0004-282X20160051erratum

ABSTRACT

There are few histomorphometric studies on the unmyelinated fibers of the fibular nerve in rats, and the number of experimental studies using this nerve has been increasing in the last years. Sixty-two percent of the endoneurial area from 10 fibular nerves of adult Wistar rats was scanned by electron microscopy, and digitized. The total number of unmyelinated axons (1.882 ± 271) was significantly lesser, and their axon diameters (0.2 μm to 2.8 μm) significantly higher than that determined in previous studies. The histogram peaked at 1 μm. The differences could be due to the nerve sampled area, the number and the age of the animals evaluated, and the laboratory techniques used. This study brings new and referential data to be used in experimental investigations involving histomorphometric evaluation of the rat fibular nerve.

RESUMO

Embora o nervo fibular de ratos venha sendo incluído progressivamente em maior número de estudos experimentais nos últimos anos, há poucos estudos a respeito das suas fibras amielínicas. Os nervos fibulares de 10 ratos Wistar adultos foram avaliados através de microscopia óptica e eletrçnica. Varredura sistemática através de microscopia eletrçnica de transmissão das áreas fasciculares da porção distal no nervo foi realizada. Em média, 62% da área endoneural foi digitalizada. O número total de axçnios amielínicos encontrados (1.882 ± 271) foi significativamente menor e as medidas dos diâmetros axonais (0,2 μm a 2,8 μm) maiores do que o determinado em estudos prévios. O pico do histograma foi constituído por fibras de 1μm. As diferenças podem ser devidas à amostragem de maior área endoneural, ao número e à idade dos animais avaliados, e as técnicas laboratoriais utilizadas. Os dados obtidos podem ser considerados referenciais para o nervo fibular de ratos Wistar adultos.

unmyelinated fibers, unmyelinated - peroneal nerve - microscopy, electron, transmission - peripheral nerves
fibras nervosas amielínicas - nervo fibular - microscopia eletrçnica de transmissão - nervos periféricos

Support:

Support: FAPESP (Processo: 2012/11012-7), CNPq (Processo: 141524/2015-4) and FAEPA (Fundação de Apoio ao Ensino, Pesquisa e à Assistência do Hospital das Clínicas da Universidade de São Paulo).




Publikationsverlauf

Eingereicht: 08. Dezember 2015

Angenommen: 16. März 2016

Artikel online veröffentlicht:
06. September 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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