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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2018; 76(04): 252-256
DOI: 10.1590/0004-282X20180019
Article

Propentofylline decreases hypothalamic astrogliosis induced by hypercaloric diet in the rat

A propentofilina diminui a astrogliose hipotalâmica induzida pela dieta hipercalórica no rato
Eduardo Fernandes Bondan
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil
2   Universidade Cruzeiro do Sul, Medicina Veterinária, São Paulo SP, Brasil
,
Carolina Cardoso Vieira
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil
,
Maria de Fátima Monteiro Martins
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil
2   Universidade Cruzeiro do Sul, Medicina Veterinária, São Paulo SP, Brasil
,
Thiago Berti Kirsten
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil
,
Maria Martha Bernardi
1   Universidade Paulista, Patologia Ambiental e Experimental, São Paulo SP, Brasil
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ABSTRACT

Obesity is associated with a chronic and low-grade inflammatory response in the hypothalamus, where astrogliosis occurs with the upregulation of the astrocyte structural protein GFAP. As propentofylline (PPF) has inhibitory effects on astrocyte and microglial activation during inflammation, this study aimed to investigate if this xanthine derivative could decrease the astrocyte reaction induced by a hypercaloric diet (HD). Male Wistar rats were divided into four groups: NDS – rats receiving a normocaloric diet (ND) and daily saline solution; NDP – rats receiving ND and daily PPF (12.5 mg/kg/day, intraperitoneal route); HDS – rats receiving HD and saline solution, HDP – rats receiving HD and PPF. On the 21st day, rats were anesthetized, and perfused, and brains were collected for GFAP immunohistochemical study in the hypothalamus. Results showed that HD induced increased weight gain and hypothalamic astrogliosis. Propentofylline decreased the expression of GFAP in the HDP group, although it did not affect the weight gain induced by this diet.

RESUMO

A obesidade está associada com uma resposta inflamatória crçnica e de baixo grau no hipotálamo, onde ocorre astrogliose com a superexpressão da proteína astrocitária GFAP. Como a propentofilina (PPF) possui efeitos inibitórios sobre a ativação astrocitária e microglial durante a inflamação, este estudo visou a investigar se esta xantina podia diminuir a reação astrocitária induzida pela dieta hipercalórica (HD). Ratos Wistar machos foram divididos em 4 grupos: NDS- ratos recebendo dieta normocalórica (ND) e solução salina diária; NDP- ratos recebendo ND e PPF diária (12.5 mg/kg/dia, via intraperitoneal); HDS- ratos recebendo HD e solução salina, HDP- ratos recebendo HD e PPF. No 21° dia, os ratos foram perfundidos e os encéfalos, coletados para estudo imuno-histoquímico para a GFAP no hipotálamo. Os resultados mostram que a HD induziu aumento do ganho de peso e astrogliose no hipotálamo. A PPF diminuiu a expressão de GFAP no grupo HD, embora não tenha afetado o ganho de peso induzido por esta dieta.

Keywords:

astrocytes - central nervous system - inflammation - obesity - xanthine

Palavras-chave:

astrócitos - sistema nervoso central - inflamação - obesidade - xantina


Publication History

Received: 27 November 2017

Accepted: 13 January 2018

Article published online:
28 August 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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