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Vet Comp Orthop Traumatol 2011; 24(05): 370-373
DOI: 10.3415/VCOT-10-09-0128
Brief Communication
Schattauer GmbH

A cryogenic clamping technique that facilitates ultimate tensile strength determinations in tendons and ligaments

J. E. Bowser
1   Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA
,
S. H. Elder
2   Department of Agricultural and Biological Engineering, Mississippi State University, Mississippi State, Mississippi, USA
,
A. M. Rashmir-Raven
3   Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA
,
C. E. Swiderski
1   Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA
› Author Affiliations Financial Support This investigation was supported by grant 07070633 from the American Quarter Horse Foundation.
Further Information

Publication History

Received: 03 September 2010

Accepted: 22 June 2011

Publication Date:
17 December 2017 (online)

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Summary

Objective: To describe the use of a cryogenic clamp of novel design for tensile strength testing of tendinous and ligamentous tissues with inherently high tensile strength.

Methods: Inexpensive, easily machined steel clamps were manufactured to facilitate rapid insertion into a standard wedge-screw grip apparatus installed on a testing system with a control system attached. The deep digital flex-or tendon (DDFT) of six horses was trimmed to a uniform dumbbell shape and secured in clamps using partial submersion in liquid nitrogen for approximately 45 seconds and immediately tested. Approximate time between removal from liquid nitrogen and failure of tendon was four minutes.

Results: Failure was achieved in all tendons tested in a region approximating a midpoint between the clamps. Ultimate failure loads of up to 6745 N were achieved without slippage of the tissue from the grips. The ultimate tensile strength of the normal equine DDFT determined in this study was 111.82 ± 11.53 N/mm2, and the stress versus grip-to-grip elongation plots for our equine DDFT were representative of a standard non-linear elastic curve obtained in similar studies.

Clinical significance: We present a low cost device for quantifying physical properties of specimens with high connective tissue concentrations and inherent high tensile strength. Results of this study indicate that this device provides a practical alternative to other more costly methods of adequately securing larger tendons and ligaments for tensile strength testing.

Keywords

Cryogenic clamp - tensile strength - deep digital flexor tendon
 

  • References

  • 1 Rincon L, Schatzmann L, Brunner P. et al Design and evaluation of a cryogenic soft tissue fixation device – Load tolerances and thermal aspects. J Biomech 2001; 34: 393-397.
    CrossrefPubMedGoogle Scholar
  • 2 Riemersma DJ, Schamhardt HC. The Cryo-jaw, a clamp designed for in vitro rheology studies of horse digital flexor tendons. J Biomech 1982; 15: 619-620.
    CrossrefPubMedGoogle Scholar
  • 3 Crevier N, Porcelot P, Denoix JM. et al Segmental variations of in vitro mechanical properties in equine superficial digital flexor tendons. Am J Vet Res 1996; 57: 1111-1117.
    PubMedGoogle Scholar
  • 4 Jansen MO, Savelberg HHCM. Stress and strain of equine tendons of the forelimb at failure. Equine Vet J 1994; S17: 57-60.
    PubMedGoogle Scholar
  • 5 Trudel G, Doherty GP, Koike Y. et al Restoration of strength despite low stress and abnormal imaging after Achilles injury. Med Sci Sports Exerc 2009; 41: 2009-2016.
    CrossrefPubMedGoogle Scholar
  • 6 Jopp I, Reese S. Morphological and bomechanical studies on the common calcaneal tendon in dogs. Vet Comp Orthop Tramatol 2009; 22: 119-124.
    PubMedGoogle Scholar
  • 7 Soslowsky LJ, Thomopoulos S, Esmail A. et al Rotator cuff tendonitis in an animal model: role of extrinsic and overuse factors. Ann Biomed Eng 2002; 30: 1057-1063.
    CrossrefPubMedGoogle Scholar