PDF herunterladen
CC BY 4.0 · AIMS Genet 2019; 06(02): 017-035
DOI: 10.3934/genet.2019.2.17
Research Article

Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress

Xiaojuan Wang
2   ChiFeng University, Agricultural Science Research Institute; Grass resource genetic breeding, China
,
Jianghong Wu
4   Inner Mongolia University for Nationalities, College of Animal Science and Technology, China
,
Zhongren Yang
1   Inner Mongolia Agricultural University, College of Grassland, Resources and Environment, Grass resource genetic breeding, China
,
Fenglan Zhang
1   Inner Mongolia Agricultural University, College of Grassland, Resources and Environment, Grass resource genetic breeding, China
,
Hailian Sun
3   Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Grass Research Center, Chinese Academy of Sciences, Grass resource genetic breeding, China
,
Xiao Qiu
3   Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Grass Research Center, Chinese Academy of Sciences, Grass resource genetic breeding, China
,
Fengyan Yi
3   Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Grass Research Center, Chinese Academy of Sciences, Grass resource genetic breeding, China
,
Ding Yang
3   Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Grass Research Center, Chinese Academy of Sciences, Grass resource genetic breeding, China
,
Fengling Shi
1   Inner Mongolia Agricultural University, College of Grassland, Resources and Environment, Grass resource genetic breeding, China
› Institutsangaben
› Weitere Informationen
   Lizenzen und Reprints

Abstract

Kochia prostrata is a good forage plant, which has important economic and ecological value in arid and semi-arid regions of China. Drought is one of the main factors affecting its productivity. At present, there are few studies on the mechanism of drought resistance. In order to reveal the changes of physiological and biochemical indexes, stomatal structure and gene expression profiles of Kochia prostrata under drought treatment, the classical determination method and high-throughput Illumina Hiseq sequencing platform were applied to the control group (CK) and drought treatment group of Kochia prostrata. The results showed that under the condition of moderate to mild drought stress, the SOD activity reached the maximum value of 350.68 U/g min on the 5th day of stress, and under the condition of severe drought stress, the SOD activity reached the maximum on the 2nd day of stress. The accumulation of Proline remained at a high level on the 5th day of stress, and there was at least one epidermal cell interval between the two adult stomatal of the leaf epidermis, so that the evaporation shell of each stomatal did not overlap, it ensures the efficient gas exchange of the stomatal, indicating that the Kochia prostrata has strong drought resistance. A total of 1,177.46 M reads were obtained by sequencing, with a total of 352.25 Gbp data and Q30 of 85%. In the differential gene annotation to the biological process (BP), a total of 261 GO terms were enriched in the up-regulated genes, and a total of 231 GO terms were enriched in the down-regulated genes. The differentially expressed genes (DEGs) were obtained in 27 KEGG metabolic pathways, which laid a foundation for revealing the molecular mechanism of drought tolerance.

Keywords

Kochia prostrate - drought stress - stomatal - transcription factors - physiological changes


Publikationsverlauf

Eingereicht: 28. Januar 2019

Angenommen: 26. Mai 2019

Artikel online veröffentlicht:
15. März 2021

© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Engelbrecht BMJ, Comita LS, Condit R. et al. Drought sensitivity shapes species distribution patterns in tropical forests. Nature 2007; 447: 80-82
    Google Scholar
  • 2 Flato G, Gregory M, Jochem A. et al. Evaluation of climate models. Climate Change. The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. 5 Eds., Cambridge University Press, Seiten, 2013; 741-866
    PubMedGoogle Scholar
  • 3 Anderegg WRL, Kane JM, Anderegg LDL. Consequences of widespread tree mortality triggered by drought and temperature stress. Nat Clim Change 2012; 3: 30-36
    Google Scholar
  • 4 Famiglietti JS, Rodell M. Environmental science. Water in the balance. Science 2013; 340: 1300-1301
    CrossrefPubMedGoogle Scholar
  • 5 Pinheiro C, Chaves MM. Photosynthesis and drought: Can we make metabolic connections from available data. J Exp Bot 2011; 62: 869-882
    CrossrefPubMedGoogle Scholar
  • 6 Davis AM. Forage quality of Prostrata Kochia compared with three browse species. Agron J 1979; 71: 822-824
    CrossrefGoogle Scholar
  • 7 Wang XJ, Sun HL, Yang D. et al. Excellent feeding plants in arid region-Kochia prostrate . Grassl Prataculture 2015; 27: 10-12
    Google Scholar
  • 8 Alata L, Zhao SY, Li JZ. The Kochia prostrate and its cultivation techniques. Anim Husb Feed Sci 2010; 31: 268-270
    Google Scholar
  • 9 Yi F, Wang XJ, Qiu X. et al. Effect of Temperature, NaCI and PEG Stress on Germination and Seedling Growth of Kochia prostrate (L.) Schrad. var.horxina D.S. Wen Seed 2016; 35: 46-50
    Google Scholar
  • 10 Hajihashemi S, Geuns JM, Ehsanpour AA. Gene transcription of steviol glycoside biosynthesis in Stevia. rebaudiana Bertoni under polyethylene glycol, paclobutrazol and gibberellic acid treatments in vitro. Acta Physiol Plant 2013; 35: 2009-2011
    CrossrefGoogle Scholar
  • 11 He Q, Zhao S, Ma Q. et al. Endogenous salicylic acid levels and signaling positively regulate Arabidopsis response to polyethylene glycol-simulated drought stress. Plant Growth Regul 2014; 33: 871-880
    CrossrefGoogle Scholar
  • 12 Abbaspour J, Ehsanpour AA. Physiological targets of salicylic acid on Artemisia aucheri BOISS as a medicinaland aromatic plant grown under in vitro drought stress. Bot Stud 2016; 57: 39
    CrossrefPubMedGoogle Scholar
  • 13 Wang X, Huang J, , Plant Physiology and Biochemistry Experiment Technology, In: Wang X, Huang J. Principles and Techniques of Plant Physiology and Biochemistry. 3 Eds, Beijing: Higher Education Press; 2015: 131-133
  • 14 Li HS. Principles and Techniques of Plant Physiology and Biochemistry Experiment 2 Eds Beijing: Higher Education Press; 2000: 46-57
  • 15 Young MD, Wakefield MJ, Smyth GK. et al. Gene ontology analysis for RNA-seq: Accounting for selection bias. Genome Biol 2010; 11: R14
    CrossrefPubMedGoogle Scholar
  • 16 Kanehisa M, Araki M, Goto S. et al. KEGG for linking genomes to life and the environment. Nucleic Acids Res 2008; 36: D480-D484
    PubMedGoogle Scholar
  • 17 Mao X, Cai T, Olyarchuk JG. et al. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics 2005; 21: 3787-3793
    CrossrefPubMedGoogle Scholar
  • 18 Chandrasekar V, Sairam RK, Srivastava GC. Physiological and biochemical responses of hexaploid and hetraploid wheat to drought tress. Agron Crop Sci 2000; 185: 219-227
    CrossrefGoogle Scholar
  • 19 McMichael BL, Elmore CD. Proline accumulation in water stressed cotton leaves. Crop Sci 1997; 17: 905-908
    Google Scholar
  • 20 Liu FL, Jensen CR, Anderson MN. et al. Drought stress effect on carbohydrate concentration in soybean leaves andpods during early reproductive development: Its implication in altering pod set. Field Crops Res 2004; 86: 1-13
    CrossrefGoogle Scholar
  • 21 Ohashi Y, Nakayama N, Saneoka H. et al. Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. Biologia Plantarum 2006; 50: 138-141
    CrossrefGoogle Scholar
  • 22 Taylor SH, Franks PJ, Hulme SP. et al. Photosynthetic pathway and ecological adaptation explain stomatal trait diversity amongst grasses. New Phytologist 2012; 193: 387-396
    CrossrefPubMedGoogle Scholar
  • 23 Franks PJ, Beerling DJ. Maximum leaf conductance driven by CO2 effects on stomatal size and density over geologic time. Proceedings of the National Academy of Sciences of the United States of America 2009; 106: 10343-10347
    CrossrefPubMedGoogle Scholar
  • 24 Liu W, Ji SX, Fang XL. et al. Protein kinase LTRPK1 influences cold adaptation and microtubule stability in rice. Plant Growth Regul 2013; 32: 483-490
    CrossrefGoogle Scholar
  • 25 Park SY, Fung P, Nishimura N. et al. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 2009; 324: 1068-1071
    CrossrefPubMedGoogle Scholar
  • 26 Zhang X, Miao YC, An GY. et al. Kannels inhibited by hydrogen peroxide mediate abscisic acid signaling in guard cells. Cell Res 2001; 11: 195-202
    CrossrefPubMedGoogle Scholar
  • 27 Rodriguez MC, Petersen M, Mundy J. Mitogen-activated protein kinase signaling in plants. Annu Rev Plant Biol 2010; 61: 621-649
    CrossrefPubMedGoogle Scholar
  • 28 Bowler C, Slooten L, Vandenbranden S. et al. Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J 1991; 10: 1723-1732
    CrossrefPubMedGoogle Scholar
  • 29 Jin H, Huang F, Cheng H. et al. Overexpression of the GmNAC2 gene, an NAC transcription factor, reduces abiotic stress tolerance in tobacco. Plant Mol Biol Rep 2013; 31: 435-442
    CrossrefGoogle Scholar
  • 30 Sun J, Hu W, Zhou R. et al. The Brachypodium distachyon BdWRKY36 gene confers tolerance to drought stress in transgenic tobacco plants. Plant Cell Rep 2015; 34: 23-35
    CrossrefPubMedGoogle Scholar
  • 31 Shafi A, Chauhan R, Gill T. et al. Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress. Plant Mol Biol 2015; 87: 615-631
    CrossrefPubMedGoogle Scholar
  • 32 Xu K, Chen S, Li T. et al. OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes. BMC Plant Biol 2015; 15: 141
    CrossrefPubMedGoogle Scholar
  • 33 Jin H, Huang F, Cheng H. et al. Overex-pression of the GmNAC2 gene, an NAC transcription factor, reduces abiotic stress tolerance in tobacco. Plant Mol Biol Rep 2015; 31: 435-442
    Google Scholar