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CC BY-NC-ND 4.0 · J Lab Physicians 2012; 4(02): 120-126
DOI: 10.4103/0974-2727.105594
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Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non-Commercial Assays: Part 3: Colorimetric Redox Indicator Assay v1.3.12

Sarman Singh
Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India
Department of Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, Stop TB Partnership, Geneva, Switzerland
,
Parveen Kumar
Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Shreya Sharma
Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Francis Mumbowa
Department of Medical Microbiology, School of Biomedical Sciences, Makerere University College of Health Sciences, Kampala, Uganda
Department of Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, Stop TB Partnership, Geneva, Switzerland
,
Anandi Martin
Department of Biochemistry and Microbiology, Ghent University, Belgium
Department of Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, Stop TB Partnership, Geneva, Switzerland
,
Nicolas Durier
Department of Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, Stop TB Partnership, Geneva, Switzerland
› Institutsangaben Source of Support: Culture and drug susceptibility testing subgroup, Stop-TB Partnership, WHO & FIND, Geneva, Switzerland.
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ABSTRACT

The previous two standard operating procedures (SOPs) related to the culture and drug susceptibility testing (DST) of Mycobacterium tuberculosis with the microscopic observation drug susceptibility assay (Part 1) and nitrate reductase assay (Part 2). The present SOP is devoted to a third non-commercial culture and DST method known as colorimetric redox indicator assay (CRI). As its name indicates, the CRI detects the ability of the M. tuberculosis to reduce the colored oxidation-reduction indicator when added to a liquid culture of M. tuberculosis, after exposing the growth to different anti-mycobacterial drugs. The change in the color of the indicator denotes the proportionate number of viable Mycobacteria in the medium. The identification and DST results can be obtained in 7-8 days. This SOP document has been developed through the culture and DST subgroup of the STOP tuberculosis (TB) Partnership New Diagnostic Working Group. It is intended for laboratories that would want to use or already use this rapid non-commercial method for culture identification and DST of M. tuberculosis, notably in resource-constraint settings in Asia and Africa.

Keywords

Mycobacterium - tuberculosis - drugs - susceptibility - testing - training


Publikationsverlauf

Artikel online veröffentlicht:
09. Mai 2020

© 2012.

Thieme Medical and Scientific Publishers Private Ltd.
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  • FURTHER READING

  • 1 Martin A, Palomino JC. Procedure manual colorimetric redox indicator (CRI) for drug susceptibility testing for M. tuberculosis [Internet], version 06-2012. Available from: http://www.tbevidence.org. [Last accessed date on 4 Feb 2012
  • 2 Martin A, Morcillo N, Lemus D, Montoro E, Telles MA, Simboli N, et al. Multicenter study of MTT and resazurin assays for testing susceptibility to first-line anti-tuberculosis drugs. Int J Tuberc Lung Dis 2005;9:901-6.
  • 3 Martin A, Portaels F, Palomino JC. Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: A systematic review and meta-analysis. J Antimicrob Chemother 2007;59:175-83.
  • 4 Sankar MM, Gopinath K, Singla R, Singh S. In-vitro antimycobacterial drug susceptibility testing of non-tubercular mycobacteria by tetrazolium microplate assay. Ann Clin Microbiol Antimicrob 2008;7:15.
  • 5 World Health Organization. Non-commercial culture and drug-susceptibility testing methods for screening of patients at risk of multi-drug resistant tuberculosis: Policy statement, July, 2010. Available from: http://www.who.int/tb/dots/laboratory/whopolicy_noncommercialculture_and_dstmethods_july10.pdf. [Last accessed date on 11 Nov. 2012]