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CC BY-NC-ND 4.0 · J Lab Physicians 2009; 1(02): 053-055
DOI: 10.4103/0974-2727.59699
Original Article

Emergence of Nonalbicans Candida in Neonatal Septicemia and Antifungal Susceptibility: Experience from a Tertiary Care Center

Nidhi Goel
Department of Microbiology, Pt. B.D.S. PGIMS, Rohtak, India
,
Prabhat K Ranjan
Department of Microbiology, Pt. B.D.S. PGIMS, Rohtak, India
,
Ritu Aggarwal
Department of Microbiology, Pt. B.D.S. PGIMS, Rohtak, India
,
Uma Chaudhary
Department of Microbiology, Pt. B.D.S. PGIMS, Rohtak, India
,
Nanda Sanjeev
Department of Pediatrics, Pt. B.D.S. PGIMS, Rohtak, India
› Institutsangaben Source of Support: Nil
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ABSTRACT

Aims: To know the distribution and antifungal susceptibility pattern of Candida species in neonatal septicemia cases.

Materials and Methods: In a prospective analysis blood samples from 825 clinically suspected cases of neonatal septicemia, collected aseptically, were cultured to look for the role of Candida spp. in septicemia. Candida isolates were speciated by germ tube test, Hi-CHROME agar, sugar fermentation, and sugar assimilation tests using standard protocol. All the Candida isolates were tested for antifungal susceptibility to fluconazole by the Disk Diffusion (DD) method and broth micro dilution-minimum inhibitory concentration (BMD-MIC) method using NCCLS guidelines.

Results: Isolation rate of Candida from neonatal septicemia cases was 8.1%. Most common isolate was C. tropicalis (61.19%), followed by C. albicans (19.40%), C. glabrata (11.94%), C. parapsilosis (5.97%) and C. guillermondii (1.49%). Low birth weight and previous antibiotic prophylaxis was found in 100% cases. Crude mortality rate was 50.1%. By DD method, 95.53% of the Candida isolates were sensitive to fluconazole. A discrepancy between DD method and BMD-MIC method was noted in 4.47% strains. One isolates each of C. tropicalis, C. albicans, and C. glabrata showed discrepancy.

Conclusion: Nonalbicans Candida has emerged as an important cause of neonatal septicemia. Routine susceptibility testing of Candida isolates by DD method should be confirmed by BMD-MIC method. Fluconazole can be used as empirical therapy for neonatal candidemia at our center.

Keywords

Antifungal sensitivity - nonalbicans Candida - neonatal septicemia


Publikationsverlauf

Artikel online veröffentlicht:
29. Januar 2020

© 2009.

Thieme Medical and Scientific Publishers Private Ltd.
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