CC BY-NC-ND 4.0 · J Lab Physicians 2009; 1(02): 067-068
DOI: 10.4103/0974-2727.59702
Original Article

Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis

RM Saldanha Dominic
Department of Microbiology, Kasturba Medical College, Mangalore, Karnataka, India
,
H V Prashanth
Department of Microbiology, K.M.C.T. Medical College, Mampetta, Kozhikode, Kerala, India
,
Shalini Shenoy
Department of Microbiology, Kasturba Medical College, Mangalore, Karnataka, India
,
Shrikala Baliga
Department of Microbiology, Kasturba Medical College, Mangalore, Karnataka, India
› Author Affiliations
Source of Support: Nil

ABSTRACT

Background: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis.

Aim: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test.

Materials and Methods: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans.

Results: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture.

Conclusion: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.



Publication History

Publication Date:
29 January 2020 (online)

© 2009.

Thieme Medical and Scientific Publishers Private Ltd.
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  • REFERENCES

  • 1 Dismukes WE. Cryptococcal meningitis in patients with AIDS. J Infect Dis 1988;157:624-8.
  • 2 Banerjee U. Cryptococcosis at AIIMS. Natl Med J India 1994;7:51-2.
  • 3 Banerjee U, Datta K, Majumdar T, Gupta K. Cryptococcosis in India: The awakening of a giant? Med Mycol 2001;39:51-67.
  • 4 Banerjee U, Datta K, Casadevall A. Serotype distribution of Cryptococcus neoformans in patients in a tertiary care centre in India. Med Mycol 2004;42:181-6.
  • 5 Banerjee U. Progress in diagnosis of opportunistic infections in HIV/AIDS. Indian J Med Res 2005;121:395-406.
  • 6 Chakrabarti A, Gupta B. Isolated detection of cryptococcal polysaccharide antigen in patients with cryptococcosis. Clin Infect Dis 1997;25:1494-5.
  • 7 Chakrabarti A, Sharma A, Sood A, Grover R, Sakhuja V, Prabhakar S, et al. Changing scenario of cryptococcosis in a tertiary care hospital in north India. Indian J Med Res 2000;112:56-60.
  • 8 Kozel TR. Virulence factors of Cryptococcus neoformans. Trends Microbiol 1995;3:295-9.
  • 9 Kwon-Drung KJ, Bennette JE, editors. Medical mycology. London: Lea and Febiger; 1992. p. 396-439.
  • 10 Chuck SL, Sande MA. Infections with Cryptococcus neoformans in acquired immunodeficiency syndrome. N Engl J Med 1989;321:794-9.
  • 11 Saha DC, Xess I, Jain N. Evaluation of conventional and serological methods for rapid diagnosis of cryptococcosis. Indian J Med Res 2008;127:483-8.
  • 12 Iyer RS, Banker DD. Cryptococcal meningitis in AIDS. Indian J Med Sci 2002;56:593-7.
  • 13 Powderly WG. Cryptococcal meningitis in HIV-infected patients. Current Infectious Disease Reports 2000;2:352-7.
  • 14 Snow RM, Dismukes WE. Cryptococcal meningitis: Diagnostic value of cryptococcal antigen in cerebrospinal fluid. Arch Intern Med 1975;135:1155-7.
  • 15 Diamond RD, Bennett JE. Prognostic factors in cryptococcal meningitis: A study in 11 cases. Ann Intern Med 1974;80:176-81.
  • 16 Gordon MA, Vedder DK. Serologic tests in diagnosis and prognosis of cryptococcosis. JAMA 1966;197:961-7.
  • 17 Gordon MA. Cryptococcal antigen test. JAMA 1981;246:1403.
  • 18 Lu H, Zhou Y, Yin Y, Pan X, Weng X. Cryptococcal antigen test revisited: Significance for cryptococcal meningitis therapy monitoring in a tertiary Chinese hospital. J Clin Microbiol 2005;43:2989-90.
  • 19 Feldmesser M, Harris C, Reichberg S, Kahn S, Casadevall A. Serum cryptococcal antigen in patients with AIDS. Clin Infect Dis 1996;23:827-30.