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CC BY-NC-ND 4.0 · J Lab Physicians 2010; 2(02): 070-073
DOI: 10.4103/0974-2727.72152
Original Article

An Approach to Uropathogenic Escherichia Coli in Urinary Tract Infections

K Prabhat Ranjan
Department of Microbiology, Pt. B D Sharma Postgraduate Institute of Medical Sciences, Rohtak, Haryana, India
,
Neelima Ranjan
Department of Microbiology, Pt. B D Sharma Postgraduate Institute of Medical Sciences, Rohtak, Haryana, India
,
Arindam Chakraborty
Kasturba Medical College, Mangalore, Karnataka,India
,
D R Arora
Maharaja Agrasen Medical College, Agroha, Hisar, Haryana, India
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Source of Support: Nil
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ABSTRACT

Purpose: To study the occurrence and characterization of Uropathogenic Escherichia coli (UPEC) in cases with urinary tract infections.

Materials and Methods: A total of 220 symptomatic cases from urinary tract infections and 50 stool samples from apparently healthy individuals were included. The colonies identified as Escherichia coli were screened for virulence factors, that is, hemolysin, Mannose Resistant and Mannose Sensitive Hemagglutination (MRHA, MSHA), Cell surface hydrophobicity, and Serum resistance.

Results: Among the 220 cases 91 (41.36%) were hemolytic, 68 (30.90%) showed MRHA, 58 (26.36%) were cell surface hydrophobicity positive, and 72 (32.72%) were serum-resistant. In 50 controls, three (6%) were hemolytic, six (12%) showed MRHA, nine (18%) showed cell surface hydrophobicity, and 12 (24%) were serum-resistant. The difference between cases and controls for hemolysis and MRHA were significant (P<0.001 and P<0.01, respectively). A total of 14 atypical E. coli were isolated from the urine and all showed the presence of one or the other virulence markers. Out of the 18 mucoid E.coli isolated, 10 were serum-resistant.

Conclusions: The present study revealed that out of 220 urinary isolates, 151 could be labeled as UPEC.

Keywords

Escherichia coli - hemolysin - hemagglutionation - serum resistance - surface hydrophobicity - virulence factor


Publication History

Article published online:
29 January 2020

© 2010.

Thieme Medical and Scientific Publishers Private Ltd.
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