Download PDF
CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(03): 366-369
DOI: 10.4103/1305-7456.184145
Original Article
Dental Investigation Society

In vitro comparison of antibacterial properties of bioceramic-based sealer, resin-based sealer and zinc oxide eugenol based sealer and two mineral trioxide aggregates

Gurpreet Singh
1   Department of Endodontics, College of Dentistry, Jazan University, Jizan, Kingdom of Saudi Arabia
,
Iti Gupta
1   Department of Endodontics, College of Dentistry, Jazan University, Jizan, Kingdom of Saudi Arabia
,
Faheim M. M. Elshamy
2   Department of Dental Materials, College of Dentistry, Jazan University, Jizan, Kingdom of Saudi Arabia
,
Nezar Boreak
1   Department of Endodontics, College of Dentistry, Jazan University, Jizan, Kingdom of Saudi Arabia
,
Husham Elraih Homeida
3   Department of Microbiology, College of Dentistry, Jazan University, Jizan, Kingdom of Saudi Arabia
› Author Affiliations
Further Information

Publication History

Publication Date:
24 September 2019 (online)

  PDF Download Permissions and Reprints

ABSTRACT

Objective: Our goal of the study was to evaluate the antibacterial properties of endodontic sealers against the E. faecalis. Materials and Methods: Six millimeters wells were made for each material in all the preinoculated petri plates. Then, the petri plates were incubated for 24 h. The zones of inhibition appeared were measured, and the measurements were put to statistical analysis. Results: EndoSequence BC Sealer, MM-mineral trioxide aggregate (MTA), and ProRoot MTA showed maximum means of diameter of zones of inhibition, whereas MM-seal and Endoseal did not show any zones of inhibition. Conclusion: EndoSequence BC Sealer was found to be a better endodontic sealer as compared to resin-based and zinc oxide-eugenol-based sealer.

Key words:

Antibacterial - endodontic sealer - EndoSequence BC Sealer - Enterococcus faecalis - mineral trioxide aggregate
 

  • REFERENCES

  • 1 Mattigatti S, Jain D, Ratnakar P, Moturi S. Antimicrobial effect of conventional root canal medicament vs. propolis against Enterococcus faecalis, staph aureus and candida albicans. J Contemp Dent Pract 2012; 13: 305-9
    CrossrefPubMedGoogle Scholar
  • 2 Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North Am population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 91: 579-86
    CrossrefPubMedGoogle Scholar
  • 3 Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32: 93-8
    CrossrefPubMedGoogle Scholar
  • 4 Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis . J Endod 2009; 35: 1051-5
    CrossrefPubMedGoogle Scholar
  • 5 Loushine BA, Bryan TE, Looney SW, Gillen BM, Loushine RJ, Weller RN. et al. Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. J Endod 2011; 37: 673-7
    CrossrefPubMedGoogle Scholar
  • 6 Nasseh AA. The rise of bioceramics. Endod Prac 2009; 8: 21-5
    Google Scholar
  • 7 Candeiro GT, Correia FC, Duarte MA, Ribeiro-Siqueira DC, Gavini G. Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer. J Endod 2012; 38: 842-5
    CrossrefPubMedGoogle Scholar
  • 8 Torabinejad M, Watson TF, Pitt FordTR. Sealing ability of a mineral trioxide aggregate when used as root end filling material. J Endod 1993; 19: 591-5
    CrossrefPubMedGoogle Scholar
  • 9 Bogen G, Kuttler S. Mineral trioxide aggregate obturation: A review and case series. J Endod 2009; 35: 777-90
    CrossrefPubMedGoogle Scholar
  • 10 Zhou HM, Du TF, Shen Y, Wang ZJ, Zheng YF, Haapasalo M. In vitro cytotoxicity of calcium silicate-containing endodontic sealers. J Endod 2015; 41: 56-61
    CrossrefPubMedGoogle Scholar
  • 11 Hegde V. Enterococcus faecalis: Clinical significance and treatment considerations. Endodontology 2009; 21: 48-52
    Google Scholar
  • 12 Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002; 35: 221-8
    CrossrefPubMedGoogle Scholar
  • 13 George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis . J Endod 2005; 31: 867-72
    CrossrefPubMedGoogle Scholar
  • 14 Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85: 86-93
    CrossrefPubMedGoogle Scholar
  • 15 Wang Z, Shen Y, Haapasalo M. Dentin extends the antibacterial effect of endodontic sealers against Enterococcus faecalis biofilms. J Endod 2014; 40: 505-8
    CrossrefPubMedGoogle Scholar
  • 16 McHugh CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro . J Endod 2004; 30: 218-9
    CrossrefPubMedGoogle Scholar
  • 17 Lovato KF, Sedgley CM. Antibacterial activity of endosequence root repair material and proroot MTA against clinical isolates of Enterococcus faecalis . J Endod 2011; 37: 1542-6
    CrossrefPubMedGoogle Scholar
  • 18 Charland T, Hartwell GR, Hirschberg C, Patel R. An evaluation of setting time of mineral trioxide aggregate and EndoSequence root repair material in the presence of human blood and minimal essential media. J Endod 2013; 39: 1071-2
    CrossrefPubMedGoogle Scholar
  • 19 Willershausen I, Wolf T, Kasaj A, Weyer V, Willershausen B, Marroquin BB. Influence of a bioceramic root end material and mineral trioxide aggregates on fibroblasts and osteoblasts. Arch Oral Biol 2013; 58: 1232-7
    CrossrefPubMedGoogle Scholar
  • 20 Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 1993; 19: 541-4
    CrossrefPubMedGoogle Scholar
  • 21 Gomes-Filho JE, Watanabe S, Bernabé PF, de Moraes CostaMT. A mineral trioxide aggregate sealer stimulated mineralization. J Endod 2009; 35: 256-60
    CrossrefPubMedGoogle Scholar
  • 22 Holland R, de Souza V, Nery MJ, Otoboni FilhoJA, Bernabé PF, Dezan JúniorE. Reaction of dogs’ teeth to root canal filling with mineral trioxide aggregate or a glass ionomer sealer. J Endod 1999; 25: 728-30
    CrossrefPubMedGoogle Scholar
  • 23 Yildirim T, Gencoglu N. Use of mineral trioxide aggregate in the treatment of large periapical lesions: Reports of three cases. Eur J Dent 2010; 4: 468-74
    Article in Thieme ConnectPubMedGoogle Scholar
  • 24 Duarte MA, Demarchi AC, Yamashita JC, Kuga MC, Fraga SdeC. pH and calcium ion release of 2 root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003; 95: 345-7
    CrossrefPubMedGoogle Scholar
  • 25 Leonardo MR, Bezerra da SilvaLA, Filho MT, Santana da SilvaR. Release of formaldehyde by 4 endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999; 88: 221-5
    CrossrefPubMedGoogle Scholar
  • 26 Pizzo G, Giammanco GM, Cumbo E, Nicolosi G, Gallina G. In vitro antibacterial activity of endodontic sealers. J Dent 2006; 34: 35-40
    CrossrefPubMedGoogle Scholar
  • 27 Slutzky-Goldberg I, Slutzky H, Solomonov M, Moshonov J, Weiss EI, Matalon S. Antibacterial properties of four endodontic sealers. J Endod 2008; 34: 735-8
    CrossrefPubMedGoogle Scholar
  • 28 Ustun Y, Sagsen B, Durmaz S, Percin D. In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis . European J Gen Dent 2013; 2: 134-8
    Google Scholar
  • 29 al-Khatib ZZ, Baum RH, Morse DR, Yesilsoy C, Bhambhani S, Furst ML. The antimicrobial effect of various endodontic sealers. Oral Surg Oral Med Oral Pathol 1990; 70: 784-90
    CrossrefPubMedGoogle Scholar
  • 30 Tabrizizadeh M, Mohammadi Z. In vitro evaluation of antibacterial activities of root canal sealers. J Clin Dent 2005; 16: 114-6
    PubMedGoogle Scholar