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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2013; 2(03): 292-295
DOI: 10.4103/2278-9626.116012
Original Article

Evaluation of cytotoxicity of six different flowable composites with the methyl tetrazolium test method

Muhammet Yalcin
Department of Restorative Dentistry, Inönü University, Faculty of Dentistry, Malatya, Turkey
,
Mustafa Ulker
1   Department of Restorative Dentistry, Selçuk University, Faculty of Dentistry, Konya, Turkey
,
Esra Ulker
1   Department of Restorative Dentistry, Selçuk University, Faculty of Dentistry, Konya, Turkey
,
Abdulkadir Sengun
2   Department of Restorative Dentistry, Kirikkale University, Faculty of Dentistry, Kirikkale, Turkey
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Abstract

Background: The aim of this study was to investigate the cytotoxicity of six different flowable composites with the methyl tetrazolium test (MTT). Materials and Methods: For MTT, six different flowable composites (Bisco Aelite, Bisco Inc., USA; Esthet X Flow, Dentsply, USA; Filtek™ Supreme XT Flowable Restorative, 3M Espe, USA; Gradia ® Direct Flo, GC, USA; Estelite ® Flow Quick, Tokuyama Dental Corporation, Japan; and Clearfil Majesty Flow, Kuraray Medical Inc., Japan) were prepared according to the manufacturer’s instructions in standard Teflon disks (2 mm × 5 mm) and the samples were extracted in 7 ml of Basal Medium Eagle with 10% new born calf serum and 5% penicillin streptomycin gentamisin for 24 h. In the experiments: The L929 cells were plated (25.000 cells/ml) in wells of 96 well-dishes and maintained in a humidified incubator for 24 h at 37°C, 5% CO2, and 95% of air. After 24 h incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with evaluates for 24 h. The cell mitochondrial activity was evaluated by the MTT. 12 well used for each specimen and MTT tests applied 2 times. The data were submitted to the statistically analyzed by one-way ANOVA and Tukey’s honestly significant difference (HSD) tests. Results: According to the results of MTT test with L-929 fibroblasts demonstrated that all freshly prepared flowable composites did not reduce vital cell numbers (P>0.05) in comparison to control group. Conclusion: This study revealed important information for clinical applications of flowable composites in dentistry.

Keywords

Flowable composites - methyltetrazolium test - toxicity


Publication History

Article published online:
01 November 2021

© 2013. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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  • References

  • 1 Wheeler JC, Woods JA, Cox MJ, Cantrell RW, Watkins FH, Edlich RF. Evolution of hydrogel polymers as contact lenses, surface coatings, dressings, and drug delivery systems. J Long Term Eff Med Implants 1996;6:207-17.
  • 2 Truffier-Boutry D, Demoustier-Champagne S, Devaux J, Biebuyck JJ, Mestdagh M, Larbanois P, et al. A physico-chemical explanation of the post-polymerization shrinkage in dental resins. Dent Mater 2006;22:405-12.
  • 3 Reichl FX, Simon S, Esters M, Seiss M, Kehe K, Kleinsasser N, et al. Cytotoxicity of dental composite (co) monomers and the amalgam component Hg (2+) in human gingival fibroblasts. Arch Toxicol 2006;80:465-72.
  • 4 Sustercic D, Cevc P, Funduk N, Pintar MM. Determination of curing time in visible-light-cured composite resins of different thickness by electron paramagnetic resonance. J Mater Sci Mater Med 1997;8:507-10.
  • 5 Michelsen VB, Moe G, Strøm MB, Jensen E, Lygre H. Quantitative analysis of TEGDMA and HEMA eluted into saliva from two dental composites by use of GC/MS and tailor-made internal standards. Dent Mater 2008;24:724-31.
  • 6 Zyrianov VV, Sumovskaya AY, Shostak AA. Application of electron spin resonance for evaluation of the level of free radicals in the myometrium in full-term pregnancy with normal labour and uterine inertia. J Biosci 2003;28:19-21.
  • 7 Tuusa SM, Puska MA, Lassila LV, Vallittu PK. Residual monomers released from glass-fibre-reinforced composite photopolymerised in contact with bone and blood. J Mater Sci Mater Med 2005;16:15-20.
  • 8 Durner J, Walther UI, Zaspel J, Hickel R, Reichl FX. Metabolism of TEGDMA and HEMA in human cells. Biomaterials 2010;31:818-23.
  • 9 Mutal L, Gani O. Presence of pores and vacuoles in set endodontic sealers. Int Endod J 2005;38:690-6.
  • 10 Milhem MM, Al-Hiyasat AS, Darmani H. Toxicity testing of restorative dental materials using brine shrimp larvae (Artemia salina). J Appl Oral Sci 2008;16:297-301.
  • 11 Park SB, Son WS, Ko CC, García-Godoy F, Park MG, Kim HI, et al. Influence of flowable resins on the shear bond strength of orthodontic brackets. Dent Mater J 2009;28:730-4.
  • 12 Schedle A, Franz A, Rausch-Fan X, Spittler A, Lucas T, Samorapoompichit P, et al. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dent Mater 1998;14:429-40.
  • 13 Wataha JC, Lockwood PE, Bouillaguet S, Noda M. In vitro biological response to core and flowable dental restorative materials. Dent Mater 2003;19:25-31.
  • 14 Wennberg A, Mjör IA, Hensten-Pettersen A. Biological evaluation of dental restorative materials - A comparison of different test methods. J Biomed Mater Res 1983;17:23-36.
  • 15 Huang TH, Tsai CY, Chen SL, Kao CT. An evaluation of the cytotoxic effects of orthodontic bonding adhesives upon a primary human oral gingival fibroblast culture and a permanent, human oral cancer-cell line. J Biomed Mater Res 2002;63:814-21.
  • 16 Jonke E, Franz A, Freudenthaler J, König F, Bantleon HP, Schedle A. Cytotoxicity and shear bond strength of four orthodontic adhesive systems. Eur J Orthod 2008;30:495-502.
  • 17 Lovell LG, Newman SM, Donaldson MM, Bowman CN. The effect of light intensity on double bond conversion and flexural strength of a model, unfilled dental resin. Dent Mater 2003;19:458-65.
  • 18 Ferracane JL. Elution of leachable components from composites. J Oral Rehabil 1994;21:441-52.
  • 19 Santerre JP, Shajii L, Leung BW. Relation of dental composite formulations to their degradation and the release of hydrolyzed polymeric-resin-derived products. Crit Rev Oral Biol Med 2001;12:136-51.
  • 20 Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: A review. Clin Oral Investig 2008;12:1-8.
  • 21 Al-Hiyasat AS, Darmani H, Milhem MM. Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clin Oral Investig 2005;9:21-5.
  • 22 Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS. Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991;12:737-40.
  • 23 Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental restorative materials. J Oral Rehabil 1994;21:453-62.