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CC BY-NC-ND 4.0 · Libyan International Medical University Journal 2020; 05(02): 27-31
DOI: 10.4103/LIUJ.LIUJ_6_20
Original Article

Sulforaphane in experimental hypertension

Ali Banigesh
Department of Pharmacology and Toxicology, College of Pharmacy, University of Benghazi, Benghazi, Libya
,
Vijitha Senanayake
1   Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, Canada
,
Salma Bukhatwa
Department of Pharmacology and Toxicology, College of Pharmacy, University of Benghazi, Benghazi, Libya
,
Bernhard Juurlink
Department of Pharmacology and Toxicology, College of Pharmacy, University of Benghazi, Benghazi, Libya
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Abstract

Background: Hypertension is defined as a failure to achieve a blood pressure (BP) target – smaller than 140/90 mmHg. The worldwide burden of hypertension has been associated with globally increased rates of death and disability. There is increasing evidence of strong relation between hypertension and oxidative stress, where either increased oxidative stress or depressed antioxidant level may lead to hypertension. Using stroke-prone spontaneously hypertensive rats (SHRSP) rats, previous studies in our laboratory have shown that broccoli sprouts (high in sulforaphane, a phase-2 protein inducer) attenuate BP and inflammation. Objectives: The question this study addressed was whether sulforaphane (a potent phase-2 protein inducer) can attenuate hypertension in the experimental model using the stroke-prone spontaneously hypertensive rats (SHRsp). Materials and Methods: Sulforaphane (LKT Laboratories) or vehicle was orally gavaged to SHRsp or Sprague–Dawley rats (SD) daily for 15 weeks. The body weight and BP were determined weekly, using a standard tail-cuff BP measurement. Tissues such as hearts and kidneys were collected, weighed, and stored under −80°C for further analysis. Results: When compared to BP in SHRsp control rats (179.9 ± 4.32), sulforaphane significantly reduced BP to 157 ± 5.21 (10 μmol/kg body weight), 136.57 ± 1.96 (20 μmol/kg body weight), and 129.33 ± 6.10 (5 μmol/kg body weight), respectively, in SHRsp rats. Conclusion: Administration of sulforaphane, a potent phase-2 enzyme inducer, daily for more than 3 months, significantly improves BP in SHRsp rats, but it did not have any effects on normotensive rats – SD.

Key-words:

Blood pressure - hypertension - SHRsp - Sprague–Dawley - sulforaphane

Financial support and sponsorship

Nil.




Publikationsverlauf

Eingereicht: 17. März 2020

Angenommen: 08. Juli 2020

Artikel online veröffentlicht:
14. Juni 2022

© 2020. Libyan International Medical University. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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