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CC BY-NC-ND 4.0 · Avicenna J Med 2018; 8(02): 46-50
DOI: 10.4103/ajm.AJM_156_17
ORIGINAL ARTICLE

Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre

Uma Chaudhary
Department of Microbiology, Pt BD Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
,
Shipra Agarwal
Department of Microbiology, Pt BD Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
,
Kausalya Raghuraman
Department of Microbiology, Pt BD Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
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Abstract

Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli, helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification of these beta lactamases.Aim: To determine the concurrent production of beta lactamases using twelve disc method on E. coli isolates.Materials and Methods: A total of 200 multidrug resistant E. coli were screened using twelve disc method. The isolates of ESBL were confirmed by ceftazidime/clavulanic acid and cefotaxime/clavulanic acid method. Metallo-beta-lactamases (MBL) were confirmed by imipenem EDTA combined disc method.Results: Among the 200 isolates, 42.5% were ESBL producers, 9% were MBL and 6.5% were Klebsiella pneumoniae carbapenemase (KPC) and AmpC each respectively. Coproduction was seen in 54 (27%). A significant difference in sensitivity was seen in cefuroxime, aztreonam, cefoxitin and ceftriaxone among inpatient and outpatients.Conclusion: The present study highlights burden of ESBL, AmpC, KPC and MBL along with their coproduction in a tertiary care hospital. In-house antibiotic policy, infection control and epidemiological surveys will help us in controlling these resistant bugs. We believe, the twelve disc method is a simple, inexpensive screening method for beta lactamase production.

Keywords

AmpC - carbapenemases - extended spectrum beta lactamases - twelve disc method


Publication History

Article published online:
12 August 2021

© 2018. Syrian American Medical Society. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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