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CC BY-NC-ND 4.0 · Eur J Dent 2017; 11(01): 126-129
DOI: 10.4103/ejd.ejd_309_16
Case Report
Dental Investigation Society

Shifts of subgingival bacterial population after nonsurgical and pharmacological therapy of localized aggressive periodontitis, followed for 1 year by Ion Torrent PGM platform

Giuseppina Campisciano
1   Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
2   Advanced Diagnostics Department, Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy
,
Annamaria Toschetti
3   Private Practice, Gorizia, Italy
,
Manola Comar
1   Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
2   Advanced Diagnostics Department, Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy
,
Rosanna Di Taranto
3   Private Practice, Gorizia, Italy
,
Federico Berton
1   Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
,
Claudio Stacchi
1   Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy
› Author Affiliations
Further Information

Publication History

Publication Date:
25 September 2019 (online)

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ABSTRACT

The possibility of targeting the hypervariable region V3 of the 16S rRNA gene using Ion Torrent Personal Genome Machine (PGM) could provide a complete analysis of subgingival plaque samples, potentially able to identify microbiological species missed by culture-based methods. A 16-year-old female smoker patient, affected by localized aggressive periodontitis, underwent a full-mouth disinfection protocol and was inserted in a 3-month recall program. Microbiological samples were collected at baseline and at 30, 100, 365 days follow-up and analyzed by Ion Torrent PGM. Capnocytophaga, Fusobacterium, Prevotella, and Treponema were the most represented pathogens at baseline. Nonsurgical treatment and systemic antibiotics drastically lowered the anaerobic species, and their presence remained limited after 100 days, while a consistent recolonization by anaerobic bacteria was detected at 365 days. The patient showed a general improvement of periodontal conditions. Differently from polymerase chain reaction and other microarray techniques, Ion Torrent performs a quantitative analysis of the microbiota, irrespective of the searched species. An accurate definition of the shifts of the bacterial community might help periodontal researchers for a better understanding of the impact of different treatment approaches or in intercepting nonresponsive conditions.

Key words:

Anaerobic bacteria - full-mouth disinfection - Ion Torrent Personal Genome Machine - localized aggressive periodontitis - nonsurgical treatment
 

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