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CC BY-NC-ND 4.0 · Libyan International Medical University Journal 2021; 06(02): 55-60
DOI: 10.4103/liuj.liuj_72_21
Original Article

Studies on use of heat in the aqueous extraction of miswak

Mohamed Sharkasi
Department of Pharmaceutical Chemistry, University of Benghazi, Benghazi, Libya
,
Mayouf Elsharif
Department of Pharmaceutical Chemistry, University of Benghazi, Benghazi, Libya
,
Abdussalam Sughir
1   Department of Pharmaceutics, Faculty of Pharmacy, University of Elmergib, Elkhums, Libya
,
Idris El-Mahdi
2   Department of Pharmaceutics, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya
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Abstract

Introduction: The antimicrobial properties of Miswak (Salvadora persica) are well documented, and the use of its extracts in the formulation of toothpastes and mouth rinses are well established. Most of the literature agrees that the organic extracts are more effective than aqueous extracts. Aims: The aim of the study was to prepare aqueous Miswak extracts using three different methods. The difference between the methods is the amount of heating used for their preparation. Furthermore, to evaluate stability during storage of such extracts, their pH, and their antimicrobial activity. Materials and Methods: Miswak extracts were prepared by maceration, infusion, and decoction methods, followed by evaluation of their extraction efficiency, stability, pH, viscosity, and antimicrobial activity. Results: A correlation was found between pH of extracts and their viscosity. The pH of extract increases to 6.5 when extensive heat was used during preparation, which is close to the normal pH of saliva and oral cavity. The accompanied increase in viscosity was an indication of increased extraction efficiency. Suppression of freezing point confirmed such observation for extracts prepared by decoction. The only positive antibacterial activity was observed for decoction extract, but it was less than that of chlorhexiden. For minimum inhibitory concentration estimation, it was found that almost 50% w/v of the extract must be used to provide the minimum microbial inhibitory effect. Conclusions: Miswak components appear to be thermostable ingredients, and the method of decoction can produce stable and effective Miswak extract.

Key-words:

Antimicrobial activity - aqueous-heat extraction - miswak

Financial support and sponsorship

Nil.




Publication History

Received: 31 May 2021

Accepted: 25 July 2021

Article published online:
14 June 2022

© 2021. Libyan International Medical University. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 Chawla HS. A new natural source for topical fluoride. J Indian Dent Assoc 1983;55:419-22.
  • 2 Chaurasia A, Patil R, Nagar A. Miswak in oral cavity-An update. J Oral Biol Craniofac Res 2013;3:98-101.
  • 3 Poureslami HR, Makarem A, Mojab F. Paraclinical effects of miswak extract on dental plaque. Dent Res J 2008;4:106-10.
  • 4 Sukkarwalla A, Ali SM, Lundberg P, Tanwir F. Efficacy of miswak on oral pathogens. Dent Res J (Isfahan) 2013;10:314-20.
  • 5 Noumi E, Snoussi M, Trabelsi N, Hajlaoui H, Ksouri R, Valentin E, et al. Antibacterial, anticandidal and antioxidant activities of Salvadora persica and Juglans regia L. extracts. J Med Plants Res 2011;5:4138-46.
  • 6 Chelli-Chentouf N, Tir Touil Meddah A, Mullié C, Aoues A, Meddah B. In vitro and in vivo antimicrobial activity of Algerian Hoggar Salvadora persica L. extracts against microbial strains from children's oral cavity. J Ethnopharmacol 2012;144:57-66.
  • 7 Al-Bagieh N, Almas K. In vitro antibacterial effects of aqueous and alcohol extracts of miswak (chewing sticks). Cairo Dent J 1997;13:221-4.
  • 8 Sofrata AH, Claesson RL, Lingström PK, Gustafsson AK. Strong antibacterial effect of miswak against oral microorganisms associated with periodontitis and caries. J Periodontol 2008;79:1474-9.
  • 9 Sofrata A, Santangelo EM, Azeem M, Borg-Karlson AK, Gustafsson A, Pütsep K. Benzyl isothiocyanate, a major component from the roots of Salvadora persica is highly active against Gram-negative bacteria. PLoS One 2011;6:e23045.
  • 10 Sinko PJ, Pharmacy SY. Pharmaceutical Sciences: Physical Chemical and Biopharmaceutical Principles in the Pharmaceutical Sciences. Walter Kluer, Netherlands, 2011:472-7.
  • 11 Darout IA, Christy AA, Skaug NI, Egeberg PK. Identification and quantification of some potentially antimicrobial anionic components in miswak extract. Indian J Pharmacol 2000;32:11-4.
  • 12 Jassoma E, Baeesa L, Sabbagh H. The antiplaque/anticariogenic efficacy of Salvadora persica (Miswak) mouthrinse in comparison to that of chlorhexidine: A systematic review and meta-analysis. BMC Oral Health 2019;19:64.
  • 13 Rahman MQ, Tejwani D, Wilson JA, Butcher I, Ramaesh K. Microbial contamination of preservative free eye drops in multiple application containers. Br J Ophthalmol 2006;90:139-41.
  • 14 Moeintaghavi A, Arab H, Khajekaramodini M, Hosseini R, Danesteh H, Niknami H. In vitro antimicrobial comparison of chlorhexidine, persica mouthwash and miswak extract. J Contemp Dent Pract 2012;13:147-52.
  • 15 Salih TM, El-Mahdi IM. The physical, chemical, and microbiological stability of chloramphenicol ophthalmic solution. Libyan Int Med Univ J 2018;3:42.
  • 16 Almas K, Stakiw JE. The effect of miswak extract from Salvadora persica stored for 18 years on microbes in vitro. Egypt Dent J 2000;46:227-30.
  • 17 Darout IA, Christy AA, Skaug NI, Egeberg PK. Identification and quantification of some potentially antimicrobial anionic components in miswak extract. Indian J Pharmacol 2000;32:11-4.
  • 18 Douglas CW, van Noort R. Control of bacteria in dental water supplies. Br Dent J 1993;174:167-74.
  • 19 Fradette L, Thomé G, Tanguy PA, Takenaka K. Power and mixing time study involving a Maxblend® impeller with viscous Newtonian and non-Newtonian fluids. Chem Eng Res Des 2007;85:1514-23.
  • 20 Batchelor CK, Batchelor GK. An Introduction to Fluid Dynamics. Cambridge University Press; 2000.
  • 21 Pal R, Rhodes E. Viscosity/concentration relationships for emulsions. J Rheol 1989;33:1021-45.
  • 22 Aframian DJ, Davidowitz T, Benoliel R. The distribution of oral mucosal pH values in healthy saliva secretors. Oral Dis 2006;12:420-3.
  • 23 Kubala E, Strzelecka P, Grzegocka M, Lietz-Kijak D, Gronwald H, Skomro P, et al. A review of selected studies that determine the physical and chemical properties of saliva in the field of dental treatment. BioMed research international. 2018. Article ID 6572381.
  • 24 Gibaldi M. Biopharmaceutics and clinical pharmacokinetics. Lea & Febiger; USA. 1991.
  • 25 Baliga S, Muglikar S, Kale R. Salivary pH: A diagnostic biomarker. J Indian Soc Periodontol 2013;17:461-5.
  • 26 Allen LV, Popovich NG, Ansel HC. Dosage form design: Pharmaceutical and formulation considerations. Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems. Lippincott Williams & Wilkins, USA. 2010:91-141.
  • 27 Glass BD, Haywood A. Stability considerations in liquid dosage forms extemporaneously prepared from commercially available products. J Pharm Pharm Sci 2006;9:398-426.
  • 28 Aulton ME, Taylor K. Pharmaceutical preformulation. In: Aulton's Pharmaceutics: The Design and Manufacture of Medicines. 4th ed. Edinburgh: Elsevie r Health Sciences; 2013.
  • 29 Mohammed SG. Comparative study of in vitro antibacterial activity of miswak extracts and different toothpastes. Am J Agric Biol Sci 2013;8:82-8.
  • 30 Carter SJ. Solutions in Cooper & Gunn's Tutorial Pharmacy. CBS Publishers & Distributors, India. 6th ed., 2005. pp 257-9.
  • 31 Pushpalatha HB, Pramod K, Sundaram R, Shyam R. Design and development of self-preserving and preservative-free herbal liquid oral formulation. J Appl Pharma Sci 2015;5:054-60.
  • 32 Al-Bagieh NH, Idowu A, Salako NO. Effect of aqueous extract of miswak on the in vitro growth of Candida albicans. Microbios 1994;80:107-13.