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CC BY-NC-ND 4.0 · Journal of Morphological Sciences 2017; 34(04): 257-261
DOI: 10.4322/jms.118617
Original Article
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Estrogen treatment effects on rats soleus muscles' glycogen content, extracellular matrix and cross-sectional area

M. T. M. Severi
1   Graduate Program of Human Movement Sciences, Methodist University of Piracicaba, Rodovia do Açúcar, Km 156, Taquaral, CEP 13400-911, Piracicaba, SP, Brazil
,
J. L. Q. Durigan
2   University of Brasília, Faculty of Ceilândia, Centro Metropolitano, Conjunto A, Lote 01, CEP 72220-275, Brasília, DF, Brazil
,
E. C. L. Pereira
2   University of Brasília, Faculty of Ceilândia, Centro Metropolitano, Conjunto A, Lote 01, CEP 72220-275, Brasília, DF, Brazil
,
L. L. Batista e Silva
2   University of Brasília, Faculty of Ceilândia, Centro Metropolitano, Conjunto A, Lote 01, CEP 72220-275, Brasília, DF, Brazil
,
W. R. Martins
2   University of Brasília, Faculty of Ceilândia, Centro Metropolitano, Conjunto A, Lote 01, CEP 72220-275, Brasília, DF, Brazil
,
E. R. Vieira
3   Florida International University, Department of Physical Therapy, 11200 SW 8th St, AHC3 - 430, University Park, 33199 - Miami, FL, USA
,
R. A. Tibana
4   Graduation Program of Physical Education, Catholic University of Brasília, Q.S. 07, Lote 01, Bloco G,Taguatinga Sul, CEP 71966-700, Brasília, DF, Brazil
,
J. Prestes
4   Graduation Program of Physical Education, Catholic University of Brasília, Q.S. 07, Lote 01, Bloco G,Taguatinga Sul, CEP 71966-700, Brasília, DF, Brazil
,
R. C. Marqueti
2   University of Brasília, Faculty of Ceilândia, Centro Metropolitano, Conjunto A, Lote 01, CEP 72220-275, Brasília, DF, Brazil
,
C. A. Silva
1   Graduate Program of Human Movement Sciences, Methodist University of Piracicaba, Rodovia do Açúcar, Km 156, Taquaral, CEP 13400-911, Piracicaba, SP, Brazil
› Author Affiliations
Further Information

Publication History

07 June 2017

05 November 2017

Publication Date:
18 October 2018 (online)

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Abstract

Objective: To evaluate the effects of estrogen treatment of rats' soleus muscles after denervation on glucose metabolism, muscle mass, glycogen content, cross-sectional area and connective tissue density. Methods: Eighteen rats were divided into the following three groups of six animals: control, denervated for 7 days (denervated), and denervated with estradiol treatment for 7 days (denervated and treated). We measured glucose and insulin tolerance, muscle glycogen, mass, cross-sectional area and connective tissue content. Results: The denervated only and the denervated and treated groups displayed a significant reduction in glucose uptake (32% and 53% respectively compared with the control group; p<0.05). Soleus muscle denervation reduced muscle glycogen (0.25 ± 0.03 vs 0.43 ± 0.02 mg/100mg; p<0.05), muscle mass (0.33 ± 0.09 vs. 0.48 ± 0.06 mg/g; p<0.05) and cross-sectional area (1626 ± 352 vs. 2234 ± 349 μm2; p<0.05), and increased connective tissue content (35 ± 7 vs. 10 ± 5%; p<0.05) compared to controls. Estrogen treatment decreased connective tissue density in the denervated and treated group (24 ± 4%; p<0.05) compared to the denervated group. It also prevented alterations on muscle glycogen in denervated and trated group. However, estrogen treatment did not prevent muscle atrophy (1626 ± 352 vs. 1712 ± 319 μm2). Conclusion: Estrogen treatment of rats' soleus muscles after denervation increased muscle glycogen content and minimized connective tissue density increase, but it did not prevent muscle atrophy.

Keywords

denervation - estrogen - muscle atrophy - muscle glycogen - connective tissue