Exp Clin Endocrinol Diabetes 2019; 127(08): 533-537
DOI: 10.1055/a-0629-9958
Methods and Techniques
© Georg Thieme Verlag KG Stuttgart · New York

Long Non-Coding RNA CASC2 Improves Diabetic Nephropathy by Inhibiting JNK Pathway

Huihui Yang
1   Department of Endocrinology, Henan Provincial People’s hospital, Zhengzhou City, Henan Province, PR. China
,
Quan e Kan
1   Department of Endocrinology, Henan Provincial People’s hospital, Zhengzhou City, Henan Province, PR. China
,
Yong Su
1   Department of Endocrinology, Henan Provincial People’s hospital, Zhengzhou City, Henan Province, PR. China
,
Hua Man
1   Department of Endocrinology, Henan Provincial People’s hospital, Zhengzhou City, Henan Province, PR. China
› Author Affiliations
Further Information

Publication History

received 17 March 2018
revised 19 April 2018

accepted 08 May 2018

Publication Date:
11 June 2018 (online)

Abstract

It’s known that long non-coding RNA CASC2 overexpression inhibit the JNK pathway in some disease models, while JNK pathway activation exacerbates diabetic nephropathy. Therefore we speculate that long non-coding RNA CASC2 can improve diabetic nephropathy by inhibiting JNK pathway. Thus, our study was carried out to investigate the involvement of CASC2 in diabetic nephropathy. We found that serum level of CASC2 was significantly lower in diabetic nephropathy patients than in normal people, and serum level of CASC2 showed no significant correlations with age, gender, alcohol consumption and smoking habits, but was correlated with course of disease. ROC curve analysis showed that serum level of CASC2 could be used to accurately predict diabetic nephropathy. Diabetes mellitus has many complications. This study also included a series of complications of diabetes, such as diabetic retinopathy, diabetic ketoacidosis, diabetic foot infections and diabetic cardiopathy, while serum level of CASC2 was specifically reduced in diabetic nephropathy. CASC2 expression level decreased, while JNK1 phosphorylation level increased in mouse podocyte cells treated with high glucose. CASC2 overexpression inhibited apoptosis of podocyte cells and reduced phosphorylation level of JNK1. We conclude that long non-coding RNA CASC2 may improve diabetic nephropathy by inhibiting JNK pathway.

 
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