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DOI: 10.1055/a-0818-3689
Catalpol Ameliorates Neointimal Hyperplasia in Diabetic Rats
Publication History
received 02 August 2018
revised 22 November 2018
accepted 03 December 2018
Publication Date:
04 January 2019 (online)
Abstract
Catalpol, an iridoid glycoside, is an isolated natural product of Rehmannia glutinosa, which has been reported to have antidiabetic properties. This study investigated the vascular protective effects of catalpol in hyperglycemic rats with balloon-injured carotid arteries. Balloon injury stress led to the upregulation of monocyte chemoattractant protein-1 expression in rats with streptozotocin-induced diabetes. Western blotting and real-time PCR were performed. In situ hybridization, immunohistochemistry, and confocal analyses were employed. Monocyte chemoattractant protein-1 levels were increased through streptozotocin induction or balloon injury. After treatment with catalpol, the neointimal hyperplasia area was reduced 2 weeks after balloon injury in hyperglycemic rats. Real-time PCR and immunohistochemical analysis demonstrated reduced levels of monocyte chemoattractant protein-1 2 weeks after the balloon injury. Monocyte chemoattractant protein-1 expression was significantly increased in balloon-injured rats compared with the control groups. Thus, treatment with catalpol affected monocyte chemoattractant protein-1 expression. This study demonstrated that catalpol downregulated monocyte chemoattractant protein-1 expression in carotid arteries and ameliorated neointimal hyperplasia in hyperglycemic rats. The suppressive effect of monocyte chemoattractant protein-1 suggests that it plays a key role in neointimal hyperplasia. The results imply that catalpol is potentially effective for preventing hyperglycemia-related ischemic cardiac diseases.
Key words
catalpol - iridoid glycoside - hyperglycemia - neointimal hyperplasia - balloon injury - MCP-1* Kou-Gi Shyu and Sheng-Wen Hou contributed equally to this work.
Supporting Information
- Supporting Information
Supplementary Fig. 1: Image of catalpol attenuated monocyte recruitment on monocyte adhesion assay with a concentration dependent manner (from 0.1 mM to 1 mM) in vascular smooth muscle cell cultures under high glucose condition. Supplementary Fig. 2: Panel A, effects of different concentration of streptozotocin on increasing blood glucose level. Panel B, effects of catalpol (5 mg/kg) on the level of blood glucose of experimental rats.
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References
- 1 Dokken BB. The pathophysiology of cardiovascular disease and diabetes: Beyond blood pressure and lipids. Diabetes Spectr 2008; 21: 160-165
- 2 Matheus AS, Tannus LR, Cobas RA, Palma CC, Negrato CA, Gomes MB. Impact of diabetes on cardiovascular disease: an update. Int J Hypertens 2013; 2013: 653789
- 3 Muhlestein JB, Anderson JL, Horne BD, Lavasani F, Allen-Maycock CA, Bair TL, Pearson RR, Carlquist JF. Effect of fasting glucose levels on mortality rate in patients with and without diabetes mellitus and coronary artery disease undergoing percutaneous coronary intervention. Am Heart J 2003; 146: 351-358
- 4 Leon BM, Maddox TM. Diabetes and cardiovascular disease: Epidemiology, biological mechanisms, treatment recommendations and future research. World J Diabetes 2015; 6: 1246-1258
- 5 Wall VZ, Bornfeldt KE. Arterial smooth muscle. Arterioscler Thromb Vasc Biol 2014; 34: 2175-2179
- 6 Lin J, Kakkar V, Lu X. Impact of MCP-1 in atherosclerosis. Curr Pharm Des 2014; 20: 4580-4588
- 7 Panee J. Monocyte chemoattractant protein 1 (MCP-1) in obesity and diabetes. Cytokine 2012; 60: 1-12
- 8 Kamei N, Tobe K, Suzuki R, Ohsugi M, Watanabe T, Kubota N, Ohtsuka-Kowatari N, Kumagai K, Sakamato K, Kobayashi M, Yamauchi T, Ueki K, Oishi Y, Nishimura S, Manabe I, Hashimoto H, Ohnishi Y, Ogata H, Tokuyama K, Tsunoda M, Ide T, Murakami K, Nagai R, Kadawaki T. Overexpression of monocyte chemoattractant protein-1 in adipose tissues causes macrophage recruitment and insulin resistance. J Biol Chem 2006; 281: 26602-26614
- 9 Si Y, Ren J, Wang P, Rateri DL, Daugherty A, Shi XD, Kent KC, Liu B. Protein kinase C-delta mediates adventitial cell migration through regulation of monocyte chemoattractant protein-1 expression in a rat angioplasty model. Arterioscler Thromb Vasc Biol 2012; 32: 943-954
- 10 Gosling J, Slaymaker S, Gu L, Tseng S, Zlot CH, Young SG, Rollins BJ, Charo IF. MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B. J Clin Invest 1999; 103: 773-778
- 11 Zhu H, Wang Y, Liu Z, Wang J, Wan D, Feng S, Yang X, Wang T. Antidiabetic and antioxidant effects of catalpol extracted from Rehmannia glutinosa (Di Huang) on rat diabetes induced by streptozotocin and high-fat, high-sugar feed. Chin Med 2016; 11: 25
- 12 Zhang X, Zhang A, Jiang B, Bao Y, Wang J, An L. Further pharmacological evidence of the neuroprotective effect of catalpol from Rehmannia glutinosa . Phytomedicine 2008; 15: 484-490
- 13 Han Y, Shen M, Tang LY, Tan G, Yang QC, Ye LH, Jiang N, Gao GP, Shao Y. Antiangiogenic effects of catalpol on rat corneal neovascularization. Mol Med Rep 2018; 17: 2187-2194
- 14 Park KS. Catalpol reduces the production of inflammatory mediators via PPAR-γ activation in human intestinal Caco-2 cells. J Nat Med 2016; 70: 620-626
- 15 Jia Y, Gong N, Li TF, Zhu B, Wang YX. Peptidic exenatide and herbal catalpol mediate neuroprotection via the hippocampal GLP-1 receptor/β-endorphin pathway. Pharmacol Res 2015; 102: 276-285
- 16 Rivellese AA, Riccardi G, Vaccaro O. Cardiovascular risk in women with diabetes. Nutr Metab Cardiovasc Dis 2010; 20: 474-480
- 17 Ku SK, Bae JS. Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo . BMB Rep 2015; 48: 519-524
- 18 Shieh JP, Cheng KC, Chung HH, Kerh YF, Yeh CH, Cheng JT. Plasma glucose lowering mechanisms of catalpol, an active principle from roots of Rehmannia glutinosa, in streptozotocin-induced diabetic rats. J Agric Food Chem 2011; 59: 3747-3753
- 19 Zhang R, Zhou J, Li M, Ma H, Qiu J, Luo X, Jia Z. Ameliorating effect and potential mechanism of Rehmannia glutinosa oligosaccharides on the impaired glucose metabolism in chronic stress rats fed with high-fat diet. Phytomedicine 2014; 21: 607-614
- 20 Huang WJ, Niu HS, Lin MH, Cheng JT, Hsu FL. Antihyperglycemic effect of catalpol in streptozotocin-induced diabetic rats. J Nat Prod 2010; 73: 1170-1172
- 21 Lu J, Ji J, Meng H, Wang D, Jiang B, Liu L, Randell E, Adeli K, Meng QH. The protective effect and underlying mechanism of metformin on neointima formation in fructose-induced insulin resistant rats. Cardiovasc Diabetol 2013; 12: 58
- 22 Guo J, Pereira TJ, Dalvi P, Yeung LSN, Swain N, Breen DM, Lam L, Dolinsky VW, Giacca A. High-dose metformin (420 mg/kg daily p.o.) increased insulin sensitivity but does not affect neointimal thickness in the rat carotid balloon injury model of restenosis. Metabolism 2017; 68: 108-118
- 23 Yan J, Wang C, Jin Y, Meng Q, Liu Q, Liu Z, Liu K, Sun H. Catalpol ameliorates hepatic insulin resistance in type 2 diabetes through acting on AMPK/NOX4/PI3K/AKT pathway. Pharmacol Res 2018; 130: 466-480
- 24 Liu YJ, Zheng CZ, Hao ZP, Zhang DJ, Mao AW, Yuan P. Catalpol ameliorates diabetic atherosclerosis in diabetic rabbits. Am J Transl Res 2016; 8: 4278-4288
- 25 Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. J Clin Invest 2005; 115: 1111-1119
- 26 Qi H, Jing Z, Xiaolin W, Changwu X, Xiaorong H, Jian Y, Jing C, Hong J. Histone demethylase JMJD2A inhibition attenuates neointimal hyperplasia in the carotid arteries of balloon-injured diabetic rats via transcriptional silencing: inflammatory gene expression in vascular smooth muscle cells. Cell Physiol Biochem 2015; 37: 719-734
- 27 Choi HJ, Jang HJ, Chung TW, Jeong SI, Cha J, Choi JY, Han CW, Jang YS, Joo M, Jeong HS, Ha KT. Catalpol suppresses advanced glycation end-products-induced inflammatory response through inhibition of reactive oxygen species in human monocytic THP-1 cells. Fitoterapia 2013; 86: 19-28
- 28 Liu JY, Zhang DJ. Amelioration by catalpol of atherosclerotic lesions in hypercholesterolemic rabbits. Planta Med 2015; 8: 175-184
- 29 Koksal B. Effect of streptozotocin on plasma insulin levels of rats and mice: A meta-analysis study. Open Access Maced J Med Sci 2015; 3: 380-383
- 30 Shyu KG, Cheng WP, Wang BW. Angiotensin II downregulates microRNA-145 to regulate kruppel-like factor 4 and myocardin expression in human coronary arterial smooth muscle cells under high glucose conditions. Mol Med 2015; 21: 616-625
- 31 Lim S, Choi SH, Shin H, Cho BJ, Park HS, Ahn BY, Kang SM, Yoon JW, Jang HC, Kim YB, Park KS. Effect of a dipeptidyl peptidase-IV inhibitor, des-fluoro-sitagliptin, on neointimal formation after balloon injury in rats. PLoS One 2012; 7: e35007
- 32 Lin CM, Chang H, Chen YH, Wu IH, Chiu JH. Wogonin inhibits IL-6-induced angiogenesis via down-regulation of VEGF and VEGFR-1, not VEGFR-2. Planta Med 2006; 72: 1305-1310
- 33 Lin CM, Wang BW, Pan CM, Fang WJ, Chua SK, Chang H, Shyu KG. Effects of flavonoids on MicroRNA 145 regulation through Klf4 and myocardin in neointimal formation in vitro and in vivo . J Nutr Biochem 2018; 52: 27-35