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Planta Med 2020; 86(13/14): 1025-1031
DOI: 10.1055/a-1197-3613
Biological and Pharmacological Activity
Original Papers

New Endophytic Fusarium spp. from Fraxinus excelsior Leaves in Slovakia

Helena Ivanova
1   Institute of Forest Ecology of the SAS, Department of Phytopathology and Mycology, Nitra, Slovak Republic
,
Lenka Malinicova
2   Institute of Biology and Ecology, Pavol Jozef Safarik University in Kosice, Kosice, Slovak Republic
,
Maria Piknova 
2   Institute of Biology and Ecology, Pavol Jozef Safarik University in Kosice, Kosice, Slovak Republic
,
Peter Pristas
2   Institute of Biology and Ecology, Pavol Jozef Safarik University in Kosice, Kosice, Slovak Republic
› Author AffiliationsSupported by: Vedecká Grantová Agentúra MŠVVaŠ SR a SAV 2/0062/18
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Abstract

Fraxinus excelsior is currently suffering from ash dieback disease caused by the fungus Hymenoscyphus fraxineus. Co-occurrence of large numbers of other fungi with endophytic as well as pathogenic lifestyles has been observed on F. excelsior. In this study, new endophytic Fusarium spp. isolate Fraxinus excelsior Fusarium from Slovak territory, associated with F. excelsior, was characterised. Using morphology-based approaches, the fungus was assigned to the genus Fusarium. The isolate formed thick-walled hyaline, fusiform, slightly curved macroconidia, produced from monophialides in sporodochia, pointed at the tip, mostly 3 – 5 septate, occasionally 6 septate, and 40 – 68 × 3.8 – 5.0 µm in size with basal pedicellate cells. For more accurate taxonomic affiliation, molecular sequence analyses of the internal transcribed spacer, translation elongation factor 1-alpha sequences, and partial β-tubulin gene were performed. While the internal transcribed spacer sequence of the isolate showed the highest similarity to the Fraxinus oxysporum species, translation elongation factor 1-alpha and partial β-tubulin sequences were distantly related to the Fraxinus avenaceum species. Based on these data, the Fraxinus excelsior Fusarium isolate could probably be considered a new species of the Fusarium genus.

Key words

Oleaceae - Fusarium - endophyte - morphology - new species - phylogenetic analyses


Publication History

Received: 07 October 2019

Accepted after revision: 06 June 2020

Article published online:
24 June 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 

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