Keywords nonsurgical rhinoplasty - nasal modeling - ASCs - nasal lipoinjection - nanofat
Introduction
Undeniably, a significant sector of patients who want to improve their facial appearance
is increasingly interested in nonsurgical procedures. This trend currently represents
a globalized perspective, not only for earlier recovery but also to quickly demonstrate
the result without so much questioning or evidence that a surgical procedure can cause
and logically with a lower cost.
The concept of nonsurgical rhinoplasty or rhinoplasty dates back to the mid-1980s.
Different materials, including bovine collagen (heterogeneous), silicone, methyl methacrylate
spheres, polytetrafluoroethylene, calcium hydroxyapatite, and hyaluronic acid (alloplastics),
have been used.[1 ] New fillers and the knowledge obtained using these materials have made some obsolete
over the years. Hyaluronic acid is probably the most widely used material as an alloplastic
rhino-modeling agent.[2 ]
Autologous facial fat injection is an excellent therapeutic resource in craniofacial
and cosmetic surgery.[3 ]
[4 ]
[5 ] Autologous fat is a suitable alternative for nasal modeling because of four factors:
ease of collection, compatibility, the temporality of the results, and safety. In
this sense, various authors have reported good results using fat grafts for nasal
modeling in cases such as radix complex, increased height of the nasal dorsum, saddle
deformity, posttraumatic sequelae, etc.[6 ]
[7 ]
For almost 20 years, in an effort to obtain higher survival rates for fat grafts,
several authors have combined autologous fat grafting with adipose-derived mesenchymal
stem cells (ASCs) for reconstructive and aesthetic purposes.[8 ]
[9 ] Traditionally, the stromal vascular fraction (SVF) is obtained through enzymatic
digestion. SVF contains different cell types: endothelial cells, pericytes, preadipocytes,
smooth muscle cells, macrophages, fibroblasts, and ASCs, all with the capacity for
specialized differentiation. A huge window of opportunity in cell therapy was quickly
visualized with this knowledge.[10 ]
The invariable and continued trend for improving facial lipofilling has led to new
techniques such as micrograft and nanograft and more competitive options to obtain
a relatively greater degree of fat grafts permanence during contour remodeling facial
rejuvenation.[9 ] These techniques have simplified enzymatic isolation by mechanically isolating the
cell elements of the SVF to obtain a higher concentration representing a more practical
and dynamic option for lipofilling. The differentiation capacity of the SVF cell conglomerate,
including ASCs, makes the entire context of facial rejuvenation and tissue regeneration
attractive and interesting.
Most autologous fat used in nonsurgical rhinoplasty is for nasal dorsum modeling.
We consider a defined and projected nasal tip, including a borderline nasolabial angle
with at least intermediate thickness skin, a priority for modeling the nasal tip.
Nasal modeling with autologous fat, enriched and not enriched with ASCs, will never
be a substitute for conventional surgical rhinoplasty. However, we have found that
a certain group of patients will not undergo surgical rhinoplasty for several reasons.
We strongly believe that we must have innovative and competitive alternatives to satisfy
the requests of our patients, who are increasingly younger.
This work describes an innovative idea for nasal modeling using micrografts enriched
with ASCs. A 21-year-old female patient came to our clinic for a nasal dorsum and
tip modeling. We describe this technique and a 12-month follow-up. Informed consent
was obtained from the patient before the procedure.
Idea
The patient is a 21-year-old woman with no relevant medical history. The procedure
was performed under local anesthesia (dental cartridge: 2% lidocaine with epinephrine
1:100,000), similar to the supraperiosteal technique used by dentists to treat upper
teeth. The needle was oriented retrolabially toward the columella base to achieve
an anesthetic effect by blocking the terminal branches of the infraorbital nerve ascending
toward the nasal tip and dorsum. Oral paracetamol and cefalexin were prescribed for
1 and 3 days after the procedure.
A tumescent and anesthetic solution of 10 mL lidocaine with 1% epinephrine in 20 mL
saline solution was prepared. The solution was infiltrated in the central zone of
the infraumbilical region. After 10 minutes, 10 mL of fat was collected using an 8-cm-long,
2.5-mm-diameter blunt-tipped multifenestrated cannula connected to a 10 mL Luer-Lok
syringe. The microapproach was closed with a small round adhesive bandage.
Fat Processing Technique
The collected fat was divided into sample 1 (5 mL) and sample 2 (5 mL). Both samples
were processed according to the Coleman technique.[11 ] The supernatant and infranatant were subsequently removed. The first (nanofat) was
used to isolate the ASCs; the second (microfat) was used for nasal modeling.
Nanofat Processing for the Isolation of Adipose-Derived Mesenchymal Stem Cells
The nanofat harvest was conducted with cannulas from Tulip Medical (San Diego, CA)
following Tonnard et al,[9 ] except we centrifuged the samples and increased the passages.
First, sample 1 was passed back and forth 40 times to another empty syringe using
a 2.4-mm transfer connector, and second, the process was repeated with a 1.2-mm transfer
connector. Third, the emulsified fat was passed once using a nanotransfer filter (500-µm
mesh). The adipocytes are mechanically destroyed by emulsification of the fat with
80 passes. Filtration through the micromesh retained the residual fibrous tissue,
isolating the cellular elements of the SVF.
Adipose-Derived Mesenchymal Stem Cells –Enriched Microfat for Nasal Modeling
Nanofat content, 2.5 mL, is slowly transferred to sample 2 using a 4-mm transfer connector
with three passes. This procedure was used to homogenize the microfat mixture with
the cellular elements of the SVF.
Rhino Modeling
A 3-mL syringe and a 10-cm-long, 1.7-mm-diameter malleable blunt-tipped cannula with
a 2-mm beveled hole at the distal end were used. Intranasally, a marginal microapproach
of 1 mm was performed lateral to the right dome. Initially, lipoinjection was performed
on the nasal dorsum in a supraperiosteal plane from distal to proximal in an amount
of 0.8 mL, which was digitally modeled to ensure uniform distribution. A 1-mm microapproach
was subsequently made into the oral mucosa 0.5 mm anterior to the upper frenulum with
the upper lip everted. The malleable cannula, previously angled at approximately 45°
degrees, was directed in the last four centimeters toward the columella base, holding
it with the thumb and forefinger, where we feel the cannula between our fingers. It
continues to be inserted safely up to the nasal tip ([Fig. 1A ]).
Fig. 1 Rhino modeling. (A ) Retrolabial introduction of the cannula angled toward the base of the columella
and nasal tip. The cannula is held between the thumb and forefinger. (B ) Lipoinjection of adipose-derived mesenchymal stem cells in the nasal tip. (C ) The nasal tip is projected 2 to 3 mm above the height of the dorsum. Lipoinjection
at the base of the columella helps maintain this increase in nasal tip projection.
We prefer to initially lipoinject 0.3 mL to control the final amount required (0.6 mL
total; [Fig. 1B ]). Specifically, the nasal tip was projected approximately 2 to 3 mm above the height
of the nasal dorsum; another 0.3 mL was injected to obtain this effect.
In this sense, 0.6 mL was also lipoinjected at the columella base to help maintain
the increase in the projection of the nasal tip ([Fig. 1C ]). We digitally model the injected fat to ensure a somewhat hyperprojected nasal
tip ([Fig. 2 ]). Finally, we place nasal tape to contain the modeling to a certain degree with
the understanding that we have injected a soft material. This tape is removed in 3
days. The results of this technique remained stable. The results were documented photographically
in a follow-up period of 3 to 12 months with no complications ([Fig. 3 ]).
Fig. 2 Finally, we digitally model the injected fat to ensure a somewhat hyperprojected
nasal tip.
Fig. 3 The sequence of the follow-up period before and after rhino modeling. (A, B, C ) Preoperative view. (D, E, F ) Postoperative view with 3 months of evolution. (G, H, I ) Postoperative view with 6 months of evolution. (J, K, L ) Postoperative view with 12 months of evolution.
Discussion
Autologous fat graft (AFG) is a good option to camouflage irregularities in the nasal
dorsum during and after surgical rhinoplasty.[12 ] In the same way, it is also a great resource to provide skin thickening, particularly
in secondary or tertiary rhinoplasties, characterized by great tissue thinness, revealing
irregularities of the cartilaginous bone framework of the nasal dorsum.[13 ]
It is important to note that we must have pertinent anatomical knowledge and follow
a safety protocol to minimize the possibility of vascular and ocular compromise. A
fundamental safety step is using a blunt-tipped cannula with a diameter larger than
blood vessels. We then perform lipoinjection slowly and retrogradely in a supraperiosteal
plane, which is mostly avascular. Lipoinjection of the nasal tip is conducted with
a cannula angled in a sub-Superficial Musculoaponeurotic System (sub-SMAS) plane.
An interesting study by Kao et al[14 ] reported that autologous fat microtransplantation is an adequate strategy to augment
the nasal dorsum with an acceptable degree of patient satisfaction after 1 to 3 sessions
and with an average follow-up period of almost 2 years. A systematic review of AFG
for augmentation rhinoplasty concluded that autologous fat rhinoplasty is a safe treatment,
with high satisfaction rates and minimal complications, 63 and 100%, respectively.[15 ]
We have not found specific information on rhinoplasty with autologous fat enriched
with ASCs. The tissue regeneration capacity of the cellular elements of SVF, within
them the ASCs, is largely due to vascular endothelial growth factor, growth factor,
and transforming growth factor b, which contribute to stem cell differentiation, in
turn favoring the proliferation of endothelial cells, blood vessels, new adipocytes,
and mesenchymal stem cells, among others.[16 ]
[17 ] We know what the unpredictability of the permanence and stability of fat grafts
represents; however, we believe that the use of ASCs at higher concentrations seems
to favor better conditions for the differentiation of the different types of cellular
elements and, therefore, obtaining relatively better graft retention rates and, logically,
greater permanence.[18 ]
[19 ]
[20 ]
In rhinoplasty with alloplastic fillers, the patient normally knows that this procedure
lasts a certain time, so it must be repeated to maintain the result. It is important
to explain to the patient the possibility of performing a second lipoinjection session
to obtain better long-term stability.
We usually establish a 6- to 9-month follow-up period where we can assess the degree
of permanence and therefore the rate of absorption; we consider that when you perform
this procedure with the ideal conditions the result will mostly be good, otherwise,
the result will not be as expected. We know the advantages of modeling the nasal dorsum
and, to a certain degree, the nasal length. However, we prioritize specific patient
conditions when modeling the nasal tip. A nasal tip with some mestizo characteristics
and thick covering tissue can be modeled, but the nasal definition and projection
improvement will be poor. Therefore, as we mentioned before, we think that the most
favorable conditions to obtain better modeling of the nasal tip are reasonably developed
and resistant lower lateral cartilages, which favor a borderline degree of definition
and projection of the nasal tip, and, logically, a skin thickness that is at least
moderately thin.
We consider that nonsurgical nasal modeling using micrografts enriched with ASCs can
be an innovative alternative. Its versatility provides advantages for aesthetic nasal
modeling and as a resource to correct other types of nasal asymmetry.
This technique will never be a substitute for surgical rhinoplasty. We believe that
we must have other options in the growing demand from patients who request nonsurgical
nasal modeling. This procedure can be performed in a minor surgery area with rapid
recovery and return to the patient's daily activities the next day. Success will depend
on whether we have the right patient, and of course, on the surgeon's experience and
creativity. It is a safe and facially reproducible option that can be performed in
less than an hour. The procedure can be repeated in case of need. Nevertheless, more
studies are needed to reinforce the stability of the long-term results.
