Abstract
X-ray-based micro-computed tomography (micro-CT) is a largely non-destructive imaging
method for the visualisation and analysis of internal structures in the ex vivo eye
and affords high resolution. In contrast to other high-resolution imaging methods,
micro-CT enables spatial recording of larger and more complex tissue structures, such
as the anterior chamber of the eye. Special contrasting methods help to enhance the
absorption properties of soft tissue, that is otherwise only weakly radiopaque. Critical
point drying (CPD), as primarily used in scanning electron microscopy, offers an additional
tool for improving differential contrast properties in soft tissue. In the visualisation
of intraosseous soft tissue, such as the efferent lacrimal ducts, sample treatment
by decalcification with ethylenediaminetetraacetic acid and subsequent CPD provides
good results for micro-CT. Micro-CT can be used for a wide range of questions in 1.
basic research, 2. application-related
studies in ophthalmology (e.g. evaluation of the preclinical application of microstents
for glaucoma treatment or analysis of the positioning of intraocular lenses) but also
3. as a supplement to ophthalmological histopathology.
Key words
anterior chamber - iridocorneal angle - lacrimal duct - micro-computed tomography
- ocular structures - critical point drying
