Horm Metab Res 2008; 40(12): 875-879
DOI: 10.1055/s-0028-1083781
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Rapid Reduction of Liver Steatosis in ω3-Depleted Rats Injected with a Novel Lipid Emulsion

Y. A. Carpentier 1 , S. Peltier 2 , L. Portois 1 , J.-L. Sebedio 3 , X. Leverve 2 , W. J. Malaisse 1
  • 1L. Deloyers Laboratory for Experimental Surgery, Brussels Free University, Brussels, Belgium
  • 2Laboratoire de Bioénergétique Fondamentale et Appliquée, INSERM E-0221, Grenoble, France
  • 3Département d’Alimentation Humaine, INRA, Clermont-Ferrand, France
Further Information

Publication History

received 11.01.2008

accepted 08.05.2008

Publication Date:
22 August 2008 (online)

Abstract

The bolus intravenous administration of a novel medium-chain triglyceride: fish oil emulsion (MCT:FO) to normal subjects was recently found to increase within 60 min the amount of long-chain polyunsaturated ω3 fatty acids (ω3) in platelet and leukocyte phospholipids and, hence, was proposed as a tool to prevent such pathological events as cardiac arrhythmia in selected patients who have to undergo urgent anesthesia and/or surgery. This study investigates whether other cells located outside the vascular bed may also benefit from this procedure for replenishing phospholipids with ω3. For such a purpose, the MCT:FO emulsion (1.0 ml) was injected into normal or ω3-depleted rats examined, one hour later, for the content and fatty acid pattern of liver triglycerides and phospholipids. Control experiments included the administration of saline or a medium-chain triglyceride:olive oil emulsion. The results reveal that the bolus intravenous injection of MCT:FO to the ω3-depleted rats resulted in the enrichment of liver phospholipids in ω3 and a marked reduction in hepatic steatosis. In conclusion, it is proposed that such a procedure may indeed allow a rapid supply of ω3 not only to circulating and vascular endothelial cells but also to extravascular cells, with a resulting correction of the biochemical and biophysical defects linked to a deficiency in these fatty acids.

References

  • 1 Simopoulos A. The importance of the ratio of omega-6/omega-3 essential fatty acids.  Biomed Pharmacother. 2002;  56 365-379
  • 2 Seo T, Blaner WS, Deckelbaum RJ. Omega-3 fatty acids: molecular approaches to optimal biological outcomes.  Curr Opin Lipidol. 2005;  16 11-18
  • 3 Billman GE, Kang JX, Leaf A. Prevention of sudden cardiac death by dietary pure ω-3 polyunsaturated fatty acids in dogs.  Circulation. 1999;  99 2452-2457
  • 4 Schrepf R, Limmert T, Weber PC, Theisen K, Sellmayer A. Immediate effects of n-3 fatty acid infusion on the induction of sustained ventricular tachycardia.  Lancet. 2004;  363 1441-1442
  • 5 Portois L, Deckelbaum RJ, Malaisse WJ, Carpentier YA. Accumulation rapide d'eicosapentaénoate dans les phospholipides cellulaires après injection intraveineuse d’une émulsion d'huile de poisson et de triglycérides à chaîne moyenne à des sujets normaux.  Nutr Clin Metab. 2004;  28 ((suppl. 1)) S53
  • 6 Peltier S, Malaisse WJ, Portois L, Demaison L, Novel-Chate V, Chardigny JM, Sebedio JL, Carpentier YA, Leverve XM. Acute in vivo administration of a fish oil-containing emulsion improves post-ischemic cardiac function in n-3 depleted rats.  Int J Mol Med. 2006;  18 471-749
  • 7 Oguzhan B, Zhang Y, Louchami K, Courtois P, Portois L, Chardigny J-M, Malaisse WJ, Carpentier YA, Sener A. Pancreatic islet function in ω3 fatty acid-depleted rats. Glucose metabolism and nutrient-stimulated insulin release.  Endocrine. 2006;  20 467-476
  • 8 Richelle M, Carpentier YA, Deckelbaum RJ. Long- and medium-chain triacylglycerols in neutral lipid-exchange process with human plasma low-density lipoproteins.  Biochemistry. 1994;  33 4872-4878
  • 9 Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipids in animal tissues.  J Biol Chem. 1957;  226 497-509
  • 10 Dahlan W, Richelle M, Kulapongse S, Rössle C, Deckelbaum RJ, Carpentier YA. Effects of essential fatty acid content of lipid emulsions on erythrocyte polyunsaturated fatty acid composition in patients on long-term parenteral nutrition.  Clin Nutr. 1992;  11 262-268
  • 11 Lepage G, Roy CC. Direct transesterification of all classes of lipids in a one step reaction.  J Lipid Res. 1986;  27 114-120
  • 12 Leyton J, Drury PJ, Crawford MA. Differential oxidation of saturated and unsaturated fatty acids in vivo in the rat.  Br J Nutr. 1987;  57 383-393
  • 13 Carpentier YA, Peltier S, Portois L, Chardigny J-M, Sebedio J-L, Leverve X, Malaisse WJ. Accelerated clearance of plasma triglycerides and free fatty acids in ω3-depleted rats.  Horm Metab Res. 2007;  39 295-305
  • 14 Oguzhan B, Sancho V, Acitores A, Villanueva-Peñacarrillo M-L, Portois L, Chardigny J-M, Sener A, Carpentier YA, Malaisse WJ. Alteration of adipocyte metabolism in ω3 fatty acid-depleted rats.  Horm Metab Res. 2006;  38 789-798
  • 15 Benatti P, Peluso G, Nicolai R, Calvani M. Polyunsaturated fatty acids: biochemical, nutritional and epigenetic properties.  J Am Coll Nutr. 2004;  23 281-302
  • 16 Davidson MH. Mechanisms for the hypotriglyceridemic effect of marine omega-3 fatty acids.  Am J Cardiol. 2006;  98 27i-33i
  • 17 Portois L, Agascioglu E, Zhang Y, Sener A, Chardigny J-M, Malaisse WJ, Carpentier YA. Rapid effects of the intravenous injection of a medium-chain triglyceride-fish oil emulsion on the triglyceride fatty acid pattern of soleus muscle from ω3 fatty acid-depleted rats.  Horm Metab Res. 2007;  39 202-206

Correspondence

W.J. Malaisse

Laboratory of Experimental Hormonology

Brussels Free University

808 Route de Lennik

1070 Brussels

Belgium

Phone: +32/2/555 62 37

Fax: +32/2/555 63 56

Email: malaisse@ulb.ac.be

    >