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Horm Metab Res 2009; 41(1): 50-54
DOI: 10.1055/s-0028-1087173
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Profile of Serum IL-1β and IL-10 Shortly after Ovariectomy and Estradiol Replacement in Rats

N. Percegoni 1 , A. C. F. Ferreira 1 , C. F. Rodrigues 1 , D. Rosenthal 1 , M. T. L. Castelo Branco 2 , V. M. Rumjanek 3 , D. P. Carvalho 1
  • 1Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
  • 2Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Brazil
  • 3Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Brazil
Further Information

Publication History

received 19.03.2008

accepted 14.07.2008

Publication Date:
07 October 2008 (online)

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Abstract

Ovariectomy leads to progressive and significant increases in body weight gain and osteoporosis and is related to changes in serum and tissue cytokine profiles, such as observed in other models of overweight. We aimed to evaluate serum interleukin-1β and interleukin-10 shortly after ovariectomy, before the establishment of overweight in rats. Female Wistar rats were submitted to ovariectomy, ovariectomy and estradiol replacement, or sham operation and compared with intact controls. Rats were killed 3, 6, 9, or 13 d after ovariectomy. Body mass and retroperitoneal fats were significant higher only 13 d after ovariectomy, and estradiol replacement to ovariectomized rats impaired both body mass and retroperitoneal fat gain. Shortly after ovariectomy (at 3 d) serum interleukin-1β levels significantly increased in ovariectomized rats, treated or not with estradiol, while serum interleukin-10 levels increased only 9 d after ovariectomy. Our results suggest the existence of an important interplay between the immune system and ovarian function. This interplay occurs regardless of significant changes in adipose tissue compartment, as ovarian excision leads to short-term changes in the pattern of interleukin-1β and interleukin-10 cytokine production that precede body weight gain and are not reverted by estradiol replacement.

Key words

castration - menopause - estrogen - adipose tissue - overweight - cytokine - immune system

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Correspondence

D. P. Carvalho

Instituto de Biofísica Carlos Chagas Filho

Universidade Federal do Rio de Janeiro

CCS-Bloco G

Cidade Universitária

Ilha do Fundão

21949-900 Rio de Janeiro

Brazil

Phone: +55/21/256 265 52

Fax: +55/21/228 081 93

Email: dencarv@biof.ufrj.br

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