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Horm Metab Res 2009; 41(5): 363-367
DOI: 10.1055/s-0028-1128145
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effect of Sibutramine on Na+, K+ ATPase Activity and Tryptophan Levels on Male and Female Rat Brain

D. C. Guzmán 1 , N. L. Ruíz 1 , E. H. García 2 , G. B. Mejía 1 , P. P. Téllez 1 , G. E. Jimenez 1 , M. De la Rosa Apreza 1 , H. J. Olguín 2 , 3
  • 1Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico City, Mexico
  • 2Laboratorio de Farmacología, INP, Mexico City, Mexico
  • 3Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City Mexico
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Publication History

received 08.08.2008

accepted 15.12.2008

Publication Date:
04 February 2009 (online)

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Abstract

Some drugs that are clinically used in weight control, like sibutramine, act on the serotonergic metabolism, but its relation with free radical (FR) production in the CNS is still unknown. The aim of the work was to evaluate the effect of sibutramine on FR production. Female and male Wistar rats (250 g weight) were used; the animals received sibutramine (10 mg/kg each 36 hours) intraperitoneally during 15 days. At the end of the study, the rats were sacrificed and their brains used to measure lipid peroxida-tion (TBARS), Na+, K+ATPase activity, reduced glutathione (GSH), and tryptophan (TRP) levels, by means of validated methods. The activity of Na+, K+ATPase and total ATPase was increased in males and decreased in females. GSH concentration was increased and the levels of TBARS decreased by an effect related to sibutramine in the female group. Sibutramine decreased TRP concentration in the female group, but increased it in the male one, with respect to the control group. Our results suggest that sibutramine produce an antioxidant effect stimulated by the endogenously produced tryptophan and it protects the fluidity of plasma membrane in rat brain.

Key words

obesity - free radicals - ATPase - tryptophan - brain

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Correspondence

H. J. Olguín

Laboratorio de Farmacología

Instituto Nacional de Pediatría

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