Abstract
The aim of this study was to determine the molecular mechanism underlying the inhibition
of vascular restenosis by Radix Sophorae Flavescentis (RSF) extract after balloon
dilatation injury. In a rat carotid model of balloon dilatation injury, the RSF extract
showed a significant inhibitory effect on vascular restenosis. Immunohistochemistry
showed that the expression of tumor necrosis factor-α (TNF-α) in the vascular focus
was markedly reduced upon treatment with the RSF extract, whereas the expression of
proliferating cell nuclear antigens (PCNA) was mostly unaffected. Colorimetric assays
of methylene blue incorporation demonstrated that the proliferation of cultured vascular
smooth muscle cells (VSMCs) was not inhibited with serum from rats treated with RSF
extract. However, the expression of TNF-α in cultured VSMCs was significantly downregulated
by serum from rats treated with RSF extract. These results suggested that RSF extract
has an inhibitory effect on vascular restenosis after balloon dilatation injury which
might be, in part, attributable to its inhibition of TNF-α expression.
Key words
Radix Sophorae Flavescentis -
Sophora flavescens Ait. - Fabaceae - vascular restenosis - TNF‐α - balloon dilatation injury
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