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Horm Metab Res 2010; 42(11): 798-802
DOI: 10.1055/s-0030-1254134
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Repeated Measurements of 11β-HSD-1 Activity in Subcutaneous Adipose Tissue from Lean, Abdominally Obese, and Type 2 Diabetes Subjects – No Change Following a Mixed Meal

M. Sjöstrand1 , P.-A. Jansson3 , J. Palming3 , J. de Schoolmeester2 , D. Gill2 , A. Rees2 , L. Sjögren3 , T. Persson1 , J. W. Eriksson1 , 3
  • 1AstraZeneca R & D, Mölndal, Sweden
  • 2AstraZeneca R & D, Alderly Park, UK
  • 3The Lundberg Laboratory for Diabetes Research, Dept of Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
Further Information

Publication History

received 26.01.2010

accepted 29.04.2010

Publication Date:
31 May 2010 (online)

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Abstract

The aim of this study was to measure 11β-HSD-1 activity in subcutaneous adipose tissue by an ex vivo method in three subgroups; lean, obese, and type 2 diabetes subjects, both in the fasting state and after a mixed meal and to determine the variability and reproducibility of this method. Eighteen subjects were investigated; 6 lean, 6 abdominally obese, and 6 type 2 diabetes subjects (BMI 22±1, 30±3 and 31±3 kg/m2, respectively). Needle biopsies were taken repeatedly and an index of 11β-HSD-1 activity was measured as percent conversion of 3H-cortisone to 3H-cortisol/100 mg tissue. For two separate biopsies taken in the fasting state on the same day, the within subjects CV was 16% and the between CV was 36% for 11β-HSD-1 activity for all subjects. For two biopsies taken in the fasting state at two different days, the total within subjects CV was 38% and the between subjects CV was 46%. Lean subjects had lower 11β-HSD-1 activity (4.8±1.5% conversion of 3H-cortisone to 3H-cortisol/100 mg tissue) than both obese (14.4±1.6% conversion, p<0.01) and type 2 diabetes subjects (11.7±1.9% conversion, p<0.05) in the fasting state. There was no effect of a meal on 11β-HSD-1 activity in any of the three groups. The conclusions from this study are: 1) the variation coefficient for the ex vivo adipose tissue 11β-HSD-1 activity method was ∼25% for repeat measures within subjects; 2) food intake had no major impact on enzyme activity; and 3) 11β-HSD-1 activity in subcutaneous adipose tissue was significantly increased in obese subjects with or without T2DM compared to lean subjects without diabetes.

Key words

11β-hydroxysteroid dehydrogenase type 1 - adipose tissue biopsies - obesity - glucose

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Correspondence

M. SjöstrandMD, PhD 

Early Phase Development

AstraZeneca R & D

431 83 Mölndal

Sweden

Fax: +46/31/706 4224

Email: mikaela.sjostrand@astrazeneca.com

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