Gender-Specific Oxidative Stress Parameters

Abstract

The aim of the present study was to examine the association of proteins that regulate iron transport/storage content and acute phase response with oxidative stress in male and female athletes. Serum ferritin, transferrin, soluble transferrin receptor, C-reactive protein, interleukin-6 and oxidative stress parameters (reactive oxygen metabolites, superoxide anion, advanced oxidation protein products, lipid hydroperoxides, superoxide-dismutase and pro-oxidant-antioxidant balance) were determined in 138 athletes (73 females and 65 males). A general linear model indicated significant gender differences between athletes in terms of reactive oxygen metabolites (307.48±61.02 vs. 276.98±50.08; P=0.030), superoxide-dismutase (114.60±41.64 vs. 101.42±38.76; P=0.001), lipid hydroperoxides (149.84±38.95 vs. 101.43±39.26; P<0.001), pro-oxidant-antioxidant balance (512.40±148.67 vs. 413.09±120.30; P=0.002), advanced oxidation protein products (1.49±0.30 vs. 0.91±0.25; P<0.001) and superoxide (2.61±0.36 vs. 2.22±0.35; P=0.001), which were all significantly higher in females. Multivariate analysis of covariance indicated gender (P<0.001), training experience (P=0.004), C-reactive protein (P=0.002), soluble transferrin receptor (P=0.004) and transferrin (P<0.001) as significant covariates. Gender accounted for the largest proportion of variability for all oxidative stress parameters (46.3%) and female athletes were more susceptible to oxidative stress. Iron transport and storage proteins (transferrin and ferritin), but also acute phase reactants, were negatively related factors for oxidative stress. In conclusion, variation in the ferritin level may contribute to the different oxidative stress level between the sexes.

free radicals - ferritin - transferrin - acute phase response - superoxide-dismutase

References

• 1 Abou-Seif MA, Youssef AA. Evaluation of some biochemical changes in diabetic patients.  Clin Chim Acta. 2004;  346 161-170
• 2 Alamdari DH, Paletas K, Pegiou T, Sarigianni M, Befani C, Koliakos G. A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients.  Clin Biochem. 2007;  40 248-254
• 3 Anraku M, Kitamura K, Shintomo R, Takeuchi K, Ikeda H, Nagano J, Ko T, Mera K, Tomita K, Otagiri M. Effect of intravenous iron administration frequency on AOPP and inflammatory biomarkers in chronic hemodialysis patients: A pilot study.  Clin Biochem. 2008;  41 1168-1174
• 4 Arosio P, Levi S. Ferritin, iron homeostasis, and oxidative damage.  Free Radic Biol Med. 2002;  33 457-463
• 5 Aslan M, Kosecik M, Horoz M, Selek S, Celik H, Erel O. Assessment of paraoxonase and arylesterase activities in patients with iron deficiency anemia.  Atherosclerosis. 2007;  191 397-402
• 6 Auclair C, Voisin E. Nitroblue tetrazolium reduction. In: Greenwald RA (eds) CRC Handbook of Methods for Oxygen Radical Research. Boca Raton FL: CRC Press; 1985: 123-132
• 7 Banfi G, Del Fabbro M. Behaviour of reticulocyte counts and immature reticulocyte fraction during a competitive season in elite athletes of four different sports.  Int J Lab Hematol. 2007;  29 127-131
• 8 Banfi G, Dolci A. Preanalytical phase of sport biochemistry and hematology.  J Sports Med Phys Fitness. 2003;  43 223-230
• 9 Biemond P, Swaak AJG, Van Eijk HG, Koster JF. Superoxide dependent iron release from ferritin in inflammatory diseases.  Free Radic Biol Med. 1988;  4 185-198
• 10 Bishop D, Edge J, Thomas C, Mercier J. Effects of high-intensity training on muscle lactate transporters and postexercise recovery of muscle lactate and hydrogen ions in women.  Am J Physiol. 2008;  295 R1991-R1998
• 11 Bogavac-Stanojevic N, Jelic-Ivanovic Z, Spasojevic-Kalimanovska V, Spasic S, Kalimanovska-Ostric D. Lipid and inflammatory markers for the prediction of coronary artery disease: A multi-marker approach.  Clin Biochem. 2007;  40 1000-1006
• 12 Bogdanis GC, Papaspyrou A, Maridaki M. Muscle metabolism and fatigue during sprint exercise: effects of creatine supplementation.  Serb J Sports Sci. 2007;  1 37-57
• 13 Cannon JG, Blumberg JB. Acute phase immune responses in exercise. In: Sen C, Packer L, Hanninen O (eds). Handbook of oxidants and antioxidants in exercise. New York: Elsevier; 2000: 177-194
• 14 Chauhan A, Chauhan V, Brown WT, Cohen I. Oxidative stress in autism: increased lipid peroxidation and reduced serum levels of ceruloplasmin and transferrin – the antioxidant proteins.  Life Sci. 2004;  75 2539-2549
• 15 Clement DB, Asmundson RC. Nutritional intake and hematological parameters in endurance runners.  Phys Sportsmed. 1982;  10 37-43
• 16 Cooper CE, Vollaard NBJ, Choueiri T, Wilson MT. Exercise, free radicals and oxidative stress.  Biochem Soc Trans. 2002;  30 280-285
• 17 Cozzi A, Santambrogio P, Levi S, Arosio A. Iron detoxifying activity of ferritin: effects of H and L human apoferritins on lipid peroxidation in vitro.  FEBS Lett. 1990;  277 119-122
• 18 Deakin V. Iron depletion in athletes. In: Burke L, Deakin V (eds). Clinical Sports Nutrition. 3rd ed. Sydney: McGraw-Hill; 2006: 263-312
• 19 Di Santolo M, Stel G, Banfi G, Gonano F, Cauci S. Anemia and iron status in young fertile non-professional female athletes.  Eur J Appl Physiol. 2008;  102 703-709
• 20 Dopsaj V, Sumarac Z, Novakovic N, Dopsaj M. Determination of parameters of iron status in evaluation of anemia in elite young Serbian water polo players.  Serb J Sports Sci. 2008;  2 91-99
• 21 Fallon KE. Screening for haematological and iron-related abnormalities in elite athletes – analysis of 576 cases.  J Sci Med Sport. 2008;  11 329-336
• 22 Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise.  Med Sci Sports Exerc. 2000;  32 1576-1581
• 23 Halliwell B, Gutteridge JMC. In: Halliwell B, Gutteridge J (eds). Free Radicals in Biology and Medicine. Oxford University Press, New York; 1999: 10-245
• 24 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
• 25 Jackson JM. Exercise and oxygen radical production by muscle. In:, Sen CK, Packer L, Hanninen O, (eds). Handbook of Oxidants and Antioxidants in Exercise. Amsterdam: Elsevier Science; 2000: 57-68
• 26 Ji LL. Antioxidants and oxidative stress in exercise.  Proc Soc Exp Biol Med. 1999;  222 283-292
• 27 Lippi G, Banfi G, Buttarello M, Ceriotti F, Daves M, Dolci A, Caputo M, Giavarina D, Montagnana M, Miconi V, Milanesi B, Mosca A, Morandini M, Salvagno GL. Recommendations for detection and management of unsuitable samples in clinical laboratories.  Clin Chem Lab Med. 2007;  45 728-736
• 28 Martinovic J, Dopsaj V, Dopsaj M, Kotur-Stevuljevic J, Vujovic A, Stefanovic A, Nesic G. Long-term effects of oxidative stress in volleyball players.  Int J Sports Med. 2009;  30 851-856
• 29 Martinović J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Stefanović A, Kasum G, Vujović A. Are oxidative stress and antioxidant defense status associated with energy expenditure in athletes of various sports?.  Serb J Sports Sci. 2010;  4 75-81
• 30 Martinovic J, Kotur-Stevuljevic J, Dopsaj V, Dopsaj M, Stefanovic A, Kasum G. Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis.  Clin Biochem. 2010;  43 1225-1229
• 31 Mastaloudis A, Leonard S, Traber M. Oxidative stress in athletes during extreme endurance exercise.  Free Rad Biol Med. 2001;  31 911-922
• 32 Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase.  J Biol Chem. 1972;  247 3170-3175
• 33 Mitchell JH, Tate C, Raven P, Cobb F, Kraus W, Moreadith R, O'Toole M, Saltin B, Wenger N. Acute response and chronic adaptation to exercise in women.  Med Sci Sports Exerc. 1992;  24 S258-S265
• 34 Nakano M, Kawanishi Y, Kamohara S, Uchida Y, Shiota M, Inatomi Y, Komori T, Miyazawa K, Gondo K, Yamasawa I. Oxidative DNA damage (8-hydroxydeoxyguanosine) and body iron status: a study on 2507 healthy people.  Free Radic Biol Med. 2003;  35 826-832
• 35 Nehlsen-Cannarella SL, Fagoaga OR, Nieman DC, Henson DA, Butterworth DE, Schmitt RL, Bailey EM, Warren BJ, Utter A, Davis JM. Carbohydrate and the cytokine response to 2.5 h of running.  J Appl Physiol. 1997;  82 1662-1667
• 36 Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK. Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans.  J Physiol. 1999;  515 287-291
• 37 Papanikolaou G, Pantopoulos K. Iron metabolism and toxicity.  Toxicol Appl Pharmacol. 2005;  202 199-211
• 38 Packer L. Oxidants, antioxidant nutrients and the athlete.  J Sports Sci. 1997;  15 353-663
• 39 Petersen AMW, Pedersen BK. The anti-inflammatory effect of exercise.  J Appl Physiol. 2005;  98 1154-1162
• 40 Proteggente AR, England TG, Rice-Evans CA, Halliwell B. Iron supplementation and oxidative damage to DNA in healthy individuals with high plasma ascorbate.  Biochem Biophys Res Commun. 2001;  288 245-251
• 41 Selmeci L, Seres L, Antal M, Lukács J, Regöly-Mérei A, Acsády G. Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated technique.  Clin Chem Lab Med. 2005;  43 294-297
• 42 Sen CK, Packer L, Hänninen O. (eds). Exercise and Oxygen Toxicity. New York: Elsevier; 1994
• 43 Sureda A, Tauler P, Aguiló A, Cases N, Fuentespina E, Cordova A, Tur JA, Pons A. Relation between oxidative stress markers and antioxidant endogenous defences during exhaustive exercise.  Free Rad Res. 2005;  39 1317-1324
• 44 Woods JA, Lu Qand, Lowder T. Exercise-induced modulation of macrophage function.  Immunol Cell Biol. 2000;  78 545-553

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