Abstract
Pediatric ischemic stroke, though relatively rare, remains an important medical problem
since 20−40% of patients have recurrent strokes and 50−85% of them suffer from long-term
neurological deficits. Approximately 20−50% of the affected children have prothrombotic
disorders, therefore upon looking for possible genetic causes of the disease we focused
on the plasminogen activator inhibitor (PAI-1) − the major inhibitor of fibrinolysis.
The aim of the present study was to investigate a possible association between the
-675_-674insG PAI-1 gene polymorphism and pediatric ischemic stroke. The study population consisted of
343 individuals: 70 children with ischemic stroke, 140 their biological parents and
133 control children. The PAI-1 gene polymorphism was genotyped using the restriction fragment length polymorphism
and was visualized by AgNO3 staining. The transmission/disequilibrium test showed exactly the same transmission
of alleles from parents to the affected children (37:37). The case-control model also
did not reveal any statistical significance in alleles and genotypes distribution
between patients and control children. The obtained results suggest that the 4 G/5 G
polymorphism of the PAI-I gene is not a risk factor of ischemic stroke in Polish children.
Key words
ischemic stroke - children - gene polymorphism - plasminogen activator inhibitor-1