MMP₋₂ Expression in Skeletal Muscle after Strength Training

Abstract

The aim of this study was to assess the effects of resistance training on ladders (RTL) on MMP₋₂ expression and blood lactate concentration [La−]. 30 male (3 months of age), albino rats were divided into 3 groups: sedentary control (SC, n=10), low resistance exercise training (Low-IntRT, n=10) and high-intensive exercise training (High-IntRT, n=10). Animals of High-IntRT were submitted to a progressively increasing overload in relation to body weight until exhaustion, while the Low-IntRT group performed the same exercise regimen with no external load. The program had a frequency of 3 times per week over 8 weeks. MMP₋₂ expression of tibialis anterior muscle and [La−] were measured. While there was a significant increase of MMP₋₂ (pro-form) in both groups, only High-IntRT significantly increased MMP₋₂ in active-form (p<0.05). Both trained groups exhibited an increase in [La−] when compared to controls, however, the increase in [La−] was significantly higher in the High-IntRT compared to Low-IntRT (p<0.05). Strong correlation was found between MMP₋₂ (active form) and [La−] in High-IntRT (r=0.91). RTL in using low and high-intensity exercise can serve as a model to demonstrate different responses of MMP₋₂ expression in an animal model. It appears active form expression of MMP₋₂ is modulated by exercise intensity.

Key words

resistance exercise - blood lactate - matrix metalopeptidases - ladder exercise

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