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Z Gastroenterol 2014; 52(1): 43-49
DOI: 10.1055/s-0033-1356362
Originalarbeit
© Georg Thieme Verlag KG Stuttgart · New York

How much Oxidative Stress Exists Without the Liver?

Wieviel oxidativer Stress entsteht ohne die Leber?
C. Thiel
1   Department of General, Visceral and Transplant Surgery, University Hospital Tuebingen
,
T. Katt
1   Department of General, Visceral and Transplant Surgery, University Hospital Tuebingen
,
M. Schenk
1   Department of General, Visceral and Transplant Surgery, University Hospital Tuebingen
,
C. Grasshoff
2   Department of Anesthesiology, University Hospital Tuebingen
,
M. H. Morgalla
3   Department or Neurosurgery, University Hospital Tuebingen
,
A. Peter
4   Division of Endocrinology, Diabetology, Angiology, Nephrology, Pathobiochemistry and Clinical Chemistry, Department of Internal Medicine, University Hospital Tuebingen
,
A. Königsrainer
1   Department of General, Visceral and Transplant Surgery, University Hospital Tuebingen
,
K. Thiel
1   Department of General, Visceral and Transplant Surgery, University Hospital Tuebingen
› Author Affiliations
Further Information

Publication History

30 October 2013

12 December 2013

Publication Date:
13 January 2014 (online)

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Abstract

Background: Oxidative stress (OS) represents an important pathogenetic factor of acute liver failure and chronic liver diseases. To elucidate whether the liver itself is a major source of OS, the present study was performed to assess OS and antioxidant status in an anhepatic porcine model.

Methods: Six pigs underwent a total hepatectomy, five pigs were sham operated. OS and antioxidant status were evaluated by measuring plasma concentrations of malondialdehyde (MDA), xanthine oxidase (XO), superoxide dismutase (SOD) and the ferric reducing ability of plasma (FRAP). They were sampled at the start of the experiment, immediately after surgery, and then at 8 and 16 hours post hepatectomy.

Results: Increased concentrations of MDA were observed in anhepatic pigs postoperatively (p < 0.02) and 8 hours after hepatectomy (p < 0.003) compared to controls. XO activity increased soon after hepatectomy (22.6 ± 5.4 mU/L versus 3.3 ± 2.1 mU/L in sham animals, p < 0.03) but returned to normal values in the further course. SOD levels did not change during the observational period in both groups. FRAP values rose significantly in the anhepatic animals compared to control (p < 0.015). A significant positive correlation was observed between MDA levels and FRAP levels (Spearman’s ρ = 0.62; p < 0.0001).

Conclusions: These findings show that hepatectomy does not completely prevent the occurrence of OS because the production and regulation of OS are also located outside the liver.

Zusammenfassung

Hintergrund: Oxidativer Stress (OS) stellt einen wichtigen pathogenetischen Faktor beim akuten Leberversagen und bei chronischen Lebererkrankungen dar. In der vorliegenden Studie wurde OS und der antioxidative Status bei einem anhepatischen Schweinemodell gemessen, um herauszufinden, ob die Leber selbst eine entscheidende Rolle bei der Entstehung von OS spielt.

Methode: 6 Schweine wurden hepatektomiert, die Kontrollgruppe bestand aus 5 Schweinen. OS und antioxidativer Status wurden bestimmt, indem die Plasmakonzentrationen von Malondialdehyd (MDA), Xanthinoxidase (XO), Superoxiddismutase (SOD) und der eisenreduzierenden antioxidativen Kapazität (FRAP) zu Beginn des Experiments, postoperativ sowie 8 und 16 Stunden nach Hepatektomie gemessen wurden.

Ergebnisse: Erhöhte MDA-Konzentrationen wurden bei den anhepatischen Tieren postoperativ (p < 0,02) und nach 8 Stunden (p < 0,003) im Vergleich zur Kontrolle beobachtet. Die XO-Aktivität nahm postoperativ zu (p < 0,03 vs. Kontrolle), kehrte jedoch im weiteren Verlauf auf Normalwerte zurück. Es fanden sich keine Veränderungen der SOD-Werte in beiden Gruppen. FRAP-Bestimmungen nahmen signifikant zu gegenüber der Kontrolle (p < 0,015). Es bestand eine positive Korrelation zwischen MDA- und FRAP-Werten (Spearman’s ρ = 0,62; p < 0,0001).

Schlussfolgerung: Diese Ergebnisse zeigen, dass das Auftreten von OS durch eine Hepatektomie nicht verhindert werden kann, da sowohl die Produktion als auch die Regulation auch außerhalb der Leber stattfinden.

Schlüsselwörter

Leber - akutes Leberversagen - Hepatektomie - oxidativer Stress

Key words

liver - acute hepatic failure - hepatectomy - oxidative stress
 

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