Redox Status of Professional Soccer Players is Influenced by Training Load Throughout a Season

Abstract

The purpose of this study was to follow-up the variation of pro-/antioxidant status throughout a whole season in elite professional soccer players from the French league (n=19, 18.3±0.6 years) and to examine a possible link between these variations and training load. 5 time points (T1, T2, T3, T4, T5) were proposed to surround crucial periods of training during the whole season: the pre-season training/mid-season periods (T1–T2 and T3–T4), the championship or in-season periods (T2–T3 and T4–T5). At these times, blood samples were collected to measure pro-/antioxidant status (in erythrocytes: the ratio of reduced glutathione/oxidized glutathione, superoxide dismutase and glutathione peroxidase activities, in plasma: alpha-tocopherol, beta-carotene), and dietary intakes were also recorded. Training loads were quantified by the rating of perceived exertion method weekly throughout the season. Pro-/antioxidant-related measurements showed no modifications except for GSH/GSSG ratio, which evolved significantly between season periods: from 36.43±4.15 (T1) to 115.99±16.43 (T2) to 91.64±21.24 (T3) to 202.29±29.26 (T4) to 59.61±14.61 (T5). We observed a significant correlation (r²=0.84) between changes in GSH/GSSH ratio and cumulated mean training loads. In conclusion, these results suggest that the redox status of professional soccer players is altered according to training period (in-season periods) and that GSH/GSSH ratio variations are correlated with cumulated training loads.

Key words

soccer - oxidative stress - longitudinal follow-up - antioxidant - RPE - Training Load

• References

• 1 Balog T, Sobocanec S, Sverko V, Krolo I, Rocić B, Marotti M, Marotti T. The influence of season on oxidant-antioxidant status in trained and sedentary subjects. Life Sci 2006; 78: 1441-1447
• 2 Bangsbo J, Mohr M, Krustrup P. Physical and metabolic demands of training and match-play in the elite football player. J Sports Sci 2006; 24: 665-674
• 3 Dellal A, Lago-Peñas C, Rey E, Chamari K, Orhant E. The effects of a congested fixture period on physical performance, technical activity and injury rate during matches in a professional soccer team. Br J Sports Med Published Online First: [02/01/2015]
• 4 Elokda AS, Nielsen DH. Effects of exercise training on the glutathione antioxidant system. Eur J Cardiovasc Prev Rehabil 2007; 14: 630-663
• 5 Elosua R, Molina L, Fito M, Arquer A, Sanchez-Quesada JL, Covas MI, Ordoñez-Llanos J, Marrugat J. Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women. Atherosclerosis 2003; 167: 327-334
• 6 Fatouros IG, Chatzinikolaou A, Douroudos II, Nikolaidis MG, Kyparos A, Margonis K, Michailidis Y, Vantarakis A, Taxildaris K, Katrabasas I, Mandalidis D, Kouretas D, Jamurtas AZ. Time-course of changes in oxidative stress and antioxidant status responses following a soccer game. J Strength Cond Res 2010; 24: 3278-3286
• 7 Faude O, Kellmann M, Ammann T, Schnittker R, Meyer T. Seasonal changes in stress indicators in high level football. Int J Sports Med 2011; 32: 259-265
• 8 Finaud J, Lac G, Filaire E. Oxidative stress: relationship with exercise and training. Sports Med 2006; 36: 327-358
• 9 Finaud J, Scislowski V, Lac GM, Durand D, Vidalin H, Robert A, Filaire E. Antioxidant status and oxidative stress in professional rugby players: evolution throughout a season. Int J Sports Med 2006; 27: 87-93
• 10 Gomez-Cabrera MC, Domenech E, Viña J. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic Biol Med 2008; 44: 126-131
• 11 Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. 4^th edition Oxford University Press; 2007
• 12 Harriss DJ, Atkinson G. Ethical standards in sports and exercise science research: 2014 update. Int J Sports Med 2013; 34: 1025-1028
• 13 Heisterberg MF, Fahrenkrug J, Krustrup P, Storskov A, Kjær M, Andersen JL. Extensive monitoring through multiple blood samples in professional soccer players. J Strength Cond Res 2013; 27: 1260-1271
• 14 Hercberg S, Preziosi P, Galan P, Devanlay M, Keller H, Bourgeois C, Potier de Courcy G, Cherouvrier F. Vitamin status of a healthy French population: dietary intakes and biochemical markers. Int J Vitam Nutr Res 1994; 64: 220-232
• 15 Impellizzeri FM, Rampinini E, Coutts AJ, Sassi A, Marcora SM. Use of RPE-based training load in soccer. Med Sci Sports Exerc 2004; 36: 1042-1047
• 16 Ispirlidis I, Fatouros IG, Jamurtas AZ, Nikolaidis MG, Michailidis I, Douroudos I, Margonis K, Chatzinikolaou A, Kalistratos E, Katrabasas I, Alexiou V, Taxildaris K. Time-course of changes in inflammatory and performance responses following a soccer game. Clin J Sport Med 2008; 18: 423-431
• 17 Knez WL, Jenkins DG, Coombes JS. The effect of an increased training volume on oxidative stress. Int J Sports Med 2014; 35: 8-13
• 18 Margonis K, Fatouros IG, Jamurtas AZ, Nikolaidis MG, Douroudos I, Chatzinikolaou A, Mitrakou A, Mastorakos G, Papassotiriou I, Taxildaris K, Kouretas D. Oxidative stress biomarkers responses to physical overtraining: implications for diagnosis. Free Radic Biol Med 2007; 43: 901-910
• 19 Marin DP, Bolin AP, Campoio TR, Guerra BA, Otton R. Oxidative stress and antioxidant status response of handball athletes: implications for sport training monitoring. Int Immunopharmacol 2013; 17: 462-470
• 20 Martin A. Apports nutritionnels conseillés pour la population française. 3ème édition Édition: 3e éd. Paris: Tec et Doc Lavoisier; 2001
• 21 Meister S, Faude O, Ammann T, Schnittker R, Meyer T. Indicators for high physical strain and overload in elite football players. Scand J Med Sci Sports 2013; 23: 156-163
• 22 Meyer T, Meister S. Routine blood parameters in elite soccer players. Int J Sports Med 2011; 32: 875-881
• 23 Nédélec M, McCall A, Carling C, Legall F, Berthoin S, Dupont G. Recovery in soccer: part I – post-match fatigue and time course of recovery. Sports Med 2012; 42: 997-1015
• 24 Pincemail J, Vanbelle S, Gaspard U, Collette G, Haleng J, Cheramy-Bien JP, Charlier C, Chapelle JP, Giet D, Albert A, Limet R, Defraigne JO. Effect of different contraceptive methods on the oxidative stress status in women aged 40–48 years from the ELAN study in the province of Liege, Belgium. Hum Reprod 2007; 22: 2335-2343
• 25 Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 2008; 88: 1243-1276
• 26 Silva JR, Ascensão A, Marques F, Seabra A, Rebelo A, Magalhães J. Neuromuscular function, hormonal and redox status and muscle damage of professional soccer players after a high-level competitive match. Eur J Appl Physiol 2013; 113: 2193-2201
• 27 Subudhi AW, Davis SL, Kipp RW, Askew EW. Antioxidant status and oxidative stress in elite alpine ski racers. Int J Sport Nutr Exerc Metab 2001; 11: 32-41
• 28 Tanskanen M, Atalay M, Uusitalo A. Altered oxidative stress in overtrained athletes. J Sports Sci 2010; 28: 309-317
• 28 Teixeira V, Valente H, Casal S, Marques F, Moreira P. Antioxidant status, oxidative stress, and damage in elite kayakers after 1 year of training and competition in 2 seasons. Appl Physiol Nutr Metab 2009; 34: 716-724
• 30 Youssef H, Groussard C, Machefer G, Minella O, Couillard A, Knight J, Gratas-Delamarche A. Comparison of total antioxidant capacity of salivary, capillary and venous samplings: interest of the salivary total antioxidant capacity on triathletes during training season. J Sports Med Phys Fitness 2008; 48: 522-529
• 31 Youssef H, Groussard C, Pincemail J, Moussa E, Jacob C, Lemoine S, Zind M, Defraigne JO, Cillard J, Delamarche P, Gratas -Delamarche A. Exercise-induced oxidative stress in overweight adolescent girls: roles of basal insulin resistance and inflammation and oxygen overconsumption. Int J Obes (Lond) 2009; 33: 447-455