Reduced High Shear Platelet Adhesion to the Vascular Media: Defective von Willebrand Factor Binding to the Interstitial Collagen

Erzsebet Komorowicz; Robert D. McBane II; Jon Charlesworth; David N. Fass

doi:10.1055/s-0037-1613077

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2002; 87(04): 763-770
DOI: 10.1055/s-0037-1613077

Review Article

Schattauer GmbH

Reduced High Shear Platelet Adhesion to the Vascular Media: Defective von Willebrand Factor Binding to the Interstitial Collagen

Erzsebet Komorowicz

¹Section of Hematology Research, Mayo Clinic and Foundation for Education and Research, Rochester, MN

⁴Visiting Scientist, from Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary

,

Robert D. McBane II

²Division of Cardiovascular Medicine, Mayo Clinic and Foundation for Education and Research, Rochester, MN

,

Jon Charlesworth

³Electron Microscopy Facility, Mayo Clinic and Foundation for Education and Research, Rochester, MN

,

David N. Fass

¹Section of Hematology Research, Mayo Clinic and Foundation for Education and Research, Rochester, MN

› Author Affiliations

Abstract

Summary

Fibrillar collagen serves as a thrombogenic surface for platelet adhesion mediated by von Willebrand factor (vWf) at high shear. Although abundant throughout the arterial wall, vWf-dependent platelet deposition to artery cross-sections from perfused citrated blood is localized to the adventitia of the vessel wall. Here we describe a similarly skewed distribution of vWf-binding sites in artery cross-sections. Binding of vWf-coated fluorescent beads, as well as detection of plasma vWf bound to artery cross-section at 3350 s⁻¹ shear rate with indirect particle-immunofluorescence or immunoelectron microscopy demonstrate vWf binding sites in the adventitia, but not in the media. A monoclonal anti-vWf antibody that interferes with vWf-binding to collagen in a microplate ELISA inhibits vWf-binding to both the adventitia and sections of collagen fibrils. Our data suggest that the media, despite its fibrillar collagen content, evidenced by electron microscopy, is defective for vWf-binding, which may explain its thromboresistant nature at high shear rates.

Keywords

Willebrand - collagen - adhesion - platelet - media

Full Text

References

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