Effect of factor VIII on tissue factor-initiated spatial clot growth

Mikhail V. Ovanesov; Elena G. Lopatina; Evgueni L. Saenko; Natalya M. Ananyeva; Ljudmila I. Ul’yanova; Olga P. Plyushch; Andrey A. Butilin; Fazly I. Ataullakhanov

doi:10.1055/s-0037-1613437

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 89(02): 235-242
DOI: 10.1055/s-0037-1613437

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Effect of factor VIII on tissue factor-initiated spatial clot growth

Authors

Mikhail V. Ovanesov

¹Laboratory of Physical Biochemistry of Blood, Russian Academy of Medical Sciences, Moscow, Russia
Elena G. Lopatina

²Haemophilia Center, Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
Evgueni L. Saenko

³Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland, USA

⁴George Washington University School of Medicine, Washington, District of Columbia, USA
Natalya M. Ananyeva

³Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland, USA
Ljudmila I. Ul’yanova

⁵Department of Genetics and Histology, Institute of Immunology, Ministry of Health, Moscow, Russia
Olga P. Plyushch

²Haemophilia Center, Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
Andrey A. Butilin

⁶Moscow State University, Moscow, Russia
Fazly I. Ataullakhanov

¹Laboratory of Physical Biochemistry of Blood, Russian Academy of Medical Sciences, Moscow, Russia

⁶Moscow State University, Moscow, Russia

Abstract

Summary

Using time-lapse videomicroscopy, we studied the role of coagulation factor VIII (fVIII) in tissue factor-initiated spatial clot growth on fibroblast monolayers in a thin layer of non-stirred recalcified plasma from healthy donors or patients with severe Haemophilia A. Analysis of temporal evolution of light-scattering profiles from a growing clot revealed existence of two phases in the clot growth-initiation phase in a narrow (0.2 mm) zone adjacent to activator surface and elongation phase in plasma volume. While the initiation phase did not differ in normal and haemophilic plasmas, the rate of clot growth in the elongation phase in haemophilic plasma constituted only 30% of that in normal plasma. Supplementation of haemophilic plasma with 0.05 U/ml fVIII restored the normal clot growth rate (44.9 ± 2.5 μm/min) at high but not at low fibroblast density. Our results indicate that the functioning of the intrinsic tenase complex is critical for normal spatial clot growth.

Keywords

Factor VIII - Haemophilia A - spatial clot growth - fibroblast - time-lapse light-scattering videomicroscopy

Full Text

References

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