Unleashing the long-distance PCR for detection of the intron 22 inversion of the factor VIII gene in severe haemophilia A

• References

• 1 Tuddenham EGD, Cooper DN. Factor VIII and haemophilia A. Oxford Monographs on Medical Genetics 1994; 25: 19-76.
• 2 Lakich D, Kazazian HH, Antonarakis SE, Gitschier J. Inversions disrupting the factor VIII gene are a common cause of severe haemophilia A. Nat Genet 1993; 5: 236-41.
• 3 Liu Q, Sommer SS. Subcycling-PCR for multiplex long distance amplification of regions with high and low GC content: application to the inversion hotspot in the FVIII gene. Biotechniques 1998; 25: 1022-8.