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Thromb Haemost 1998; 79(05): 1021-1024
DOI: 10.1055/s-0037-1615113
Review Article
Schattauer GmbH

Human Platelet CD36 (GPIIIb, GPIV) Binds to Cholesteryl-Hemisuccinate and Can Be Purified by a Simple Two-Step Method Making Use of this Property

A. Kronenberg
1   From the Department of Internal Medicine, University of Münster, Münster, Germany
,
H. Grahl
1   From the Department of Internal Medicine, University of Münster, Münster, Germany
,
B. Kehrel
1   From the Department of Internal Medicine, University of Münster, Münster, Germany
› Author Affiliations
Further Information

Publication History

Received 19 July 1996

Accepted after resubmission 21 January 1998

Publication Date:
07 December 2017 (online)

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Summary

CD36, also known as GPIIIb or GPIV, is a main protein of the platelet membrane. Accumulating evidence implicates CD36 in the pathogenesis of atherosclerosis. CD36 binds to cholesteryl-hemisuccinate coupled to agarose. This binding may be of physiologic relevance since CD36 has a long extracellular hydrophobic region at its N-terminus and belongs to a family of proteins involved in lipid metabolism. This newly discovered binding property of CD36 was used to develop a rapid, efficient and gentle two-step purification method for CD36 from human platelets.

 

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