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Nuklearmedizin 1962; 2(01): 1-20
DOI: 10.1055/s-0037-1621168
Originalarbeiten — Original Articles — Travaux Originaux
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Simultaneous Studies with Fe59 and Cr51 in Congenital Haemolytic Anaemias

B. Malamos
1   Department of Clinical Therapeutics, University of Athens and Radioisotope Laboratory, Alexandra Hospital, Athens
,
E. H. Belcher
1   Department of Clinical Therapeutics, University of Athens and Radioisotope Laboratory, Alexandra Hospital, Athens
,
E. Gyftaki
1   Department of Clinical Therapeutics, University of Athens and Radioisotope Laboratory, Alexandra Hospital, Athens
,
D. Binopoulos
1   Department of Clinical Therapeutics, University of Athens and Radioisotope Laboratory, Alexandra Hospital, Athens
› Author Affiliations
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Publication History

Publication Date:
09 January 2018 (online)

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Summary

Double tracer methods using Fe59 and Cr51 for simultaneous clinical investigations of erythropoiesis and red cell destruction are described.

In these methods, the clearance of intravenously injected Fe59 from the plasma and its incorporation into the red cells is followed by the assay of blood samples, whilst the turnover of Fe59 by the tissues is followed by body surface counting. At the same time, the survival of Cr51-labelled red cells in the circulating is followed by the assay of blood samples whilst sites of destruction of labelled cells are identified by body surface counting. Pulse height analysis techniques are used for simultaneous measurements of Fe59 and Cr51 both in vitro and in vivo.

The use of these techniques is illustrated by results obtained in studies on normal subjects and on patients with various congenital haemolytic anaemias.

Résumé

On décrit une méthode à indicateur double Fe59 et Cr51 pour l’étude simultanée de l’érythropoèse et de la destruction des globules rouges.

Par cette méthode la clearance plasmique du Fe59 injecté par voie intraveineuse et son incorporation dans les globules sont déterminées par la mesure de l’activité dans des échantillons de sang, tandis que le métabolisme du Fe59 est déterminé par la mesure de l’activité à la surface. En même temps la survie des globules marqués par Cr51 dans la circulation sanguine est mesurée dans des échantillons de sang, et l’endroit de leur destruction est déterminé par un mesurage à la surface du corps. La méthode de spectrométrie de l’énergie est utilisée pour compter simultanément du Fe59 et du Cr51 in vitro comme in vivo.

L’application de cette méthode est démonstrée à l’aide de résultats de sujets normaux et de malades, souffrants de divers anémies hémolytiques.

Zusammenfassung

Es wird die Verwendung von Fe59 und Cr51 zur gleichzeitigen klinischen Untersuchung der Erythropoese und des Erythrozytenabbaus beschrieben.

Bei dieser Methode wird die Verschwinderate des intravenös injizierten Fe59 aus dem Plasma und sein Einbau in die Erythrozyten mittels Radioaktivitätsbestimmung von Blutproben verfolgt, während der Umsatz des Fe59 im Gewebe durch Körperoberflächenmessungen bestimmt wird. Gleichzeitig wird die Überlebensdauer von Cr51-markierten Erythrozyten im Kreislauf in Blutproben gemessen und der Ort ihres Abbaus durch Körperoberflächenmessungen festgestellt. Die Methode der Impulshöhenanalyse wird zur gleichzeitigen Zählung des Fe59 und Cr51 sowohl in vitro als auch in vivo verwendet.

Die Anwendungsmöglichkeiten dieser Methoden werden an Hand von Ergebnissen bei Normalpersonen und bei Patienten mit verschiedenen angeborenen hämolytischen Anämien aufgezeigt.

 

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