Download PDF
Nuklearmedizin 1971; 10(02): 146-149
DOI: 10.1055/s-0038-1624743
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Immunofluorescent Tests in the Course of 99mTc Scintiscanning Procedures in Children and Adolescents

L’examen immunofluoroscopique pendant la scintigraphic à l’aide du 99mTc chez les enfants et les jeunesImmunfluoreszenz-Reaktion nach 99mTc-Testung aus diagnostischen Gründen bei jungen Menschen
Wojciech Jeske
1   From the Department of Thyroid Diseases, Medical Clinic I (Head: Prof. W. Hartwig) and the Department of Pathology (Head: Prof. M. Kobuszewska-Faryna) Postgraduate Medical School, Warsaw, Poland
,
Tadeusz Górowski
1   From the Department of Thyroid Diseases, Medical Clinic I (Head: Prof. W. Hartwig) and the Department of Pathology (Head: Prof. M. Kobuszewska-Faryna) Postgraduate Medical School, Warsaw, Poland
,
Kazimiera Gajl-Peczalska
1   From the Department of Thyroid Diseases, Medical Clinic I (Head: Prof. W. Hartwig) and the Department of Pathology (Head: Prof. M. Kobuszewska-Faryna) Postgraduate Medical School, Warsaw, Poland
,
Oskar Chomicki
1   From the Department of Thyroid Diseases, Medical Clinic I (Head: Prof. W. Hartwig) and the Department of Pathology (Head: Prof. M. Kobuszewska-Faryna) Postgraduate Medical School, Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Received: 03 March 1971

Publication Date:
24 January 2018 (online)

    Permissions and Reprints

Summary

A group of 18 girls and 2 boys scintiscanned with 99mTc were tested for the presence of thyroid antibodies by the indirect Coons method and the passive haemagglutination test. Before the 99mTc administration no antibodies were detected, but 6 to 14 weeks later antibodies directed against the second colloid antigen (CA-2) appeared in 9 cases. They did not last and after 3 to 4 months no antibodies could be found.

Les anticorps thyroïdiens ont été déterminés à l’aide de la méthode indirecte de Coons et du test passif de l’haemagglutination chez 18 jeunes filles et 2 garçons, chez qui la scintigraphic thyroïdienne (99mTc) avait été exécutée auparavant.

Avant l’administration du 99mTc les anticorps n’étaient pas présents; or, chez 9 sujets dans 6 à 14 semaines après l’examen scintigraphique on a trouvé l’apparition des anticorps contre le 2ème antigène colloïdal. Dans un délai de 3 à 4 mois ces anticorps ont disparus.

In einer Gruppe von 18 Mädchen und 2 Knaben, die für einen 99mTc-Test vorgesehen waren, wurden immunologische Untersuchungen mit der Coons-Methode (Immunofluoreszenz) und der passiven Hämagglutinationsmethode durchgeführt .

Vor der 99mTc-Testung wurden im Serum keine Antikörper gefunden, aber 6—1 4 Wochen nach der 99mTc-Testung wurden bei 9 Personen Antikörper, wahrscheinlich gegen CA-2, festgestellt. Diese Reaktionen waren vorübergehend und nach weiteren 3—4 Monaten waren im Serum keine Antikörper mehr feststellbar.

 

  • References

  • 1 Balfour B. et al Fluorescent antibody studies in human thyroiditis; autoantibodies to an antigen of thyroid colloid distinct from thyroglobuline. Brit. J. exp. Path 1961; 42: 307.
    PubMedGoogle Scholar
  • 2 Coons A, Kaplan M. Localisation of antigen in tissue cells. II. Improvement in the method for detection of antigen by means of fluorescent antibody. J. exp. Med 1950; 91: 1.
    PubMedGoogle Scholar
  • 3 Fulthorpe A. et al Astable sheep cell preparation for detecting thyroglobuline autoantibodies and its clinical application. J. clin. Path 1961; 14: 654.
    CrossrefPubMedGoogle Scholar
  • 4 Gorowski T. et al Thyroid Scanning with 99mTc in Children and Adolescents. Nucl. Med. (Stuttg.) 1970; 9: 218.
    PubMedGoogle Scholar
  • 5 Halberg P. et al. Focal and diffuse thyroiditis and thyroid antibodies. Acta path,microbiol. scand 1968; 73: 49.
    PubMedGoogle Scholar
  • 6 Massaiski W. et al. Immunofluorescent tests during administration of 131 . J. Pol. med. J 1965; 4: 1238.
    Google Scholar
  • 7 Smith E. M. Internal Dose Calculation for “99mTc. J. nucl. Med 1964; 6: 231.
    Google Scholar