CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S15
DOI: 10.1055/s-0038-1639767
Abstracts
Aerodigestivtrakt: Aerodigestive tract

Preclinical analyses of the cell-assisted lipotransfer (CAL) for injection laryngoplasty

K Radeloff
1   Universitäts-HNO-Klinik Oldenburg, Oldenburg
,
NE Tecle
2   HNO-Universitätsklnik Würzburg, Würzburg
,
A Radeloff
1   Universitäts-HNO-Klinik Oldenburg, Oldenburg
,
N Kleinsasser
3   HNO-Universitätsklinik Linz, Linz, Österreich
,
R Hagen
4   HNO-Universitätsklinik Würzburg, Würzburg
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    The enrichment of adipose tissue with adipose-derived stromal cells (ASCs) or cells of the stromal vascular fraction (SVF) is suggested to enhance the stability of the implanted fat graft. In order to improve the stability of fat tissue injection laryngoplasty using CAL, cell-seeded fat grafts with different concentrations of ASCs were analyzed in an in vivo model.

    From the adipose tissue of the neck of 14 New Zealand White Rabbits ASCs were isolated and cultivated in vitro. In a further step adipose tissue was harvested, minced and mixed with three different concentrations of labelled ASCs. Subsequently the cell-lipografts were injected subcutaneously into the auricle of the rabbits. Adipose tissue without cells served as control. After 1, 3 and 12 weeks the implants were harvested. The volume of the implants was determined and the tissue was analyzed using histological and immunhistochemical techniques.

    During 3 months after implantation there was a distinct volume reduction in all animals without significant differences between the treatment groups. Especially the constructs containing high ASC concentrations showed more areas of necrosis in the histological examinations. This results is also reflected by the immunhistochemical staining for perilipin, which revealed reduced areas of intact adipocytes during the time course.

    In this study we did not find evidence for an advantage of CAL regarding the long-term stability of the fat grafts' volume. This may be the result of an extensive processing of the injected adipose tissue.


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    No conflict of interest has been declared by the author(s).

    Dr. Katrin Radeloff
    Universitäts-HNO-Klinik Oldenburg,
    Steinweg 13 – 17, 26122,
    Oldenburg

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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