Open Access
CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S110
DOI: 10.1055/s-0038-1640094
Abstracts
Onkologie: Oncology

Exosomes from HPV-positive and HPV-negative HNC cell lines: novel option as liquid biomarker?

S Ludwig
1   Universitätsklinik Essen, Essen
,
M Pietrowska
2   Maria Sklodowska-Curie Insitute-Oncology Center, Gliwice, Poland
,
L Marczak
3   Institute of Bioorganic Chemistry, Polish Academy of Science, Poznan, Poland
,
A Abramowicz
2   Maria Sklodowska-Curie Insitute-Oncology Center, Gliwice, Poland
,
M Gawin
2   Maria Sklodowska-Curie Insitute-Oncology Center, Gliwice, Poland
,
P Sharma
4   Department of Pathology, University of Pittsburgh School of Medicine and Univers, Pittsburgh, USA
,
MN Theodoraki
5   HNO-Klinik, Universitätsklinik Ulm, Ulm
,
S Lang
6   HNO-Klinik, Universitätsklinik Essen, Essen
,
P Widlak
2   Maria Sklodowska-Curie Insitute-Oncology Center, Gliwice, Poland
,
T Whiteside
4   Department of Pathology, University of Pittsburgh School of Medicine and Univers, Pittsburgh, USA
› Author Affiliations
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    Introduction:

    Exosomes, virus-sized vesicles, carry protein cargos resembling those of their parent cells and modify immune cell functions. Here, we address the query whether exosomes derived from Human Papilloma Virus-positive (HPV+) or HPV-negative (HPV-) HNC cell lines differ in molecular and immunoregulatory profiles.

    Methods:

    Concentrated supernatants of 3 HPV+ (SCC-2, SCC-47, SCC-90) and 2 HPV- (PCI-13, PCI-30) HNC cell lines were added to Sepharose-based size exclusion chromatography columns. Exosomes in fraction #4 were assessed for morphology and size by electron microscopy (TEM) and for protein content by BCA. Protein profiles were determined by Western blots and mass spectrometry. The impact of exosomes on immune functions were measured in co-culture assays with human T cells.

    Results:

    Exosomes from all cell lines were similar in size (30 – 150nm) and protein levels (2 – 10ug/mL). HPV+ cell line and exosomes carried E6, E7 and p16. Immunomodulating molecules (TGFß, FasL, HSP70) were equally expressed on all exosomes. Mass spectrometry and gene ontology analysis revealed that driving energy pathways were twice frequent in HPV+ exosomes (p < 0.0001), whereas cell growth/maintenance and immune response were higher expressed in HPV- exosomes. No differences were observed in the ability of HPV+ vs. HPV- exosomes to induce apoptosis in activated T cells or suppress their activation and proliferation.

    Conclusions:

    Exosomes derived from HPV+ HNCs carried viral antigens and had proteomic profiles that were distinct from that of HPV- exosomes. Immunosuppression mediated by HPV+ and HPV- exosomes were comparable. Exosomes reflect the HPV status of their parent cells and thus might serve as future plasma biomarkers of HPV infection in HNC.


     

    No conflict of interest has been declared by the author(s).

    Dr. Sonja Ludwig
    Universitätsklinik Essen,
    Hufelandstr. 55, 45147,
    Essen

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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